Pairwise croissance de la concurrence analyse pour déterminer la condition physique réplication du virus de l'immunodéficience humaines
11,087 Views
•
11:19 min
•
May 04, 2015
DOI:
Summary
Automatically generated
Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.
Vidéos Connexes
10:18
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Vidéos Connexes
11952 Views
11:05
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
Vidéos Connexes
9385 Views
N/A
Next-generation Viral RNA/DNA in situ Hybridization Applications in Human Immunodeficiency Virus/Simian Immunodeficiency Virus Research
Vidéos Connexes
2046 Views
05:46
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
Vidéos Connexes
17740 Views
14:23
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Vidéos Connexes
15526 Views
12:03
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
Vidéos Connexes
7771 Views
10:34
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Vidéos Connexes
7446 Views
11:14
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Vidéos Connexes
9048 Views
07:22
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
Vidéos Connexes
6322 Views
14:27
CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
Vidéos Connexes
9731 Views
Read Article
Cite this Article
Manocheewa, S., Lanxon-Cookson, E. C., Liu, Y., Swain, J. V., McClure, J., Rao, U., Maust, B., Deng, W., Sunshine, J. E., Kim, M., Rolland, M., Mullins, J. I. Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses. J. Vis. Exp. (99), e52610, doi:10.3791/52610 (2015).
Copy