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Neuroscience

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JoVE Journal Neuroscience
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
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Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Article DOI: 10.3791/53825-v • 10:53 min • February 26th, 2018
February 26th, 2018 •
*1, *1, 1, 1, 2, 1, 1, 1
1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute
* These authors contributed equally

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résumé
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This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.

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Brain Slices N-Methyl-D-glucamine Optimized Method Patch Clamp Electrophysiology Synaptic Properties Neuronal Preservation Patch Clamp Recording Adult Mouse Brain Tissue Slicing Station Tissue Slicer Machine Surgical Instruments Zirconium Ceramic Injector Blade Specimen Holder NMDG HEPES ACSF Brain Slice Recovery Chamber
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