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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Chapitres
- 00:00Titre
- 00:54Construction of ADE2 or LUC1 Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
- 03:06Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based ADE2 Yeast Assay
- 04:27Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based LUC1 Yeast Assay
- 07:06Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
- 08:08Résultats
- 13:51Conclusion
Presented here are four protocols to construct and exploit yeast Saccharomyces cerevisiae reporter strains to study human P53 transactivation potential, impacts of its various cancer-associated mutations, co-expressed interacting proteins, and the effects of specific small molecules.
