JoVE Journal
Biochemistry
Biochemistry
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.