Design and Implementation of a Rat <em>Ex Vivo</em> Lung Perfusion Model

William J. Cleveland; Josephine E. Hees; Claudius Balzer; Hunter F. Douglas; Troy Stevens; Matthias  L. Riess

doi:10.3791/64740

Conception et mise en œuvre d’un modèle de perfusion pulmonaire ex vivo chez le rat

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Biology

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JoVE Journal Biology

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model

Conception et mise en œuvre d’un modèle de perfusion pulmonaire ex vivo chez le rat

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04:38 min

May 26, 2023

DOI: 10.3791/64740-v

William J. Cleveland*¹, Josephine E. Hees*^1,2, Claudius Balzer^1,2, Hunter F. Douglas¹, Troy Stevens^3,4,5, Matthias L. Riess^1,6,7

¹Anesthesiology,Vanderbilt University Medical Center, ²Anesthesiology,University of Greifswald, ³Department of Physiology and Cell Biology,University of South Alabama, ⁴Department of Medicine,University of South Alabama, ⁵Center for Lung Biology,University of South Alabama, ⁶Anesthesiology,Tennessee Valley Healthcare System Veterans Affairs Medical Center, ⁷Pharmacology,Vanderbilt University

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Overview

This study presents a cost-effective and modular ex vivo lung setup designed for various experimental designs. It allows researchers to investigate lung physiology in a controlled environment, thus complementing in vivo studies in elucidating novel mechanisms.

Key Study Components

Research Area

Ex vivo lung physiology
Cardiopulmonary resuscitation (CPR) outcomes
Modular experimental setups

Background

Importance of studying lung behavior outside living organisms
Limitations of commercial setups
Need for affordable experimental methods

Methods Used

DIY surgical procedures for lung isolation
Rat model for ex vivo lung studies
Tracheostomy, cannulation, and perfusion techniques

Main Results

Successful implementation of a modular lung setup
Maintenance of lung viability post ischemia and reperfusion
Ability to conduct basic physiological measurements

Conclusions

The developed method provides a versatile tool for lung research.
Facilitates low-cost experiments that contribute to understanding respiratory function.

Frequently Asked Questions

What is the purpose of the ex vivo lung setup?

To conduct lung studies in a controlled environment, complementing in vivo experiments.

How does this method improve upon existing setups?

It is more affordable and highly modular, allowing for adaptation to different research goals.

What are the risks during the surgical procedure?

Main risks include damaging the lungs or causing excessive bleeding.

Can this setup be used for other experimental purposes?

Yes, it is versatile and can be tailored for various scientific inquiries.

What skills are needed to execute the procedure?

A basic understanding of respiratory anatomy and surgical techniques is required.

How long can the lungs remain viable after ischemia?

Lungs remained viable for up to 150 minutes after an ischemic period of eight minutes.

What measurements can be performed post-experiment?

Basic measurements include wet/dry weight and histological analysis.

Les poumons ex vivo sont utiles pour une variété d’expériences afin de recueillir des données physiologiques tout en excluant les variables confondantes des expériences in vivo. Les configurations commerciales sont souvent coûteuses et limitées dans les types de données qu’elles peuvent collecter. Nous décrivons une méthode de construction d’une configuration entièrement modulaire, adaptable à divers modèles d’étude.

Nous démontrons une méthode simple pour créer et mettre en œuvre une configuration pulmonaire ex vivo à partir de zéro. Cela permet une configuration abordable et modulaire qui peut être mise en œuvre pour une variété de configurations. La technique présentée ici est abordable, modulaire et adaptable aux laboratoires qui mettent en place le protocole.

L’opération ex vivo peut être utilisée pour compléter les modèles in vivo afin d’aider à élucider de nouveaux mécanismes. Cette configuration de bricolage est conçue pour étudier les poumons dans un environnement contrôlé. Il s’aligne sur l’objectif principal de la recherche de notre laboratoire, qui est d’étudier diverses approches pour améliorer les résultats après la RCP.

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