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Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays

Article DOI: 10.3791/65115-v • 04:00 min • May 3rd, 2024
May 3rd, 2024 •
1,2, 2,3, 2,3, 2,3, 2,3, 1,4, 3, 3, 3, 1,2,3,4,5,6
1Electrical and Computer Engineering, Johns Hopkins University, 2HEPIUS Innovation Laboratory, Johns Hopkins University School of Medicine, 3Neurosurgery, Johns Hopkins University School of Medicine, 4Biomedical Engineering, Johns Hopkins University School of Medicine, 5Mechanical Engineering, Johns Hopkins University, 6Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine

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Here, we present a protocol for using a high-throughput system that enables the monitoring and quantification of the neuromodulatory effects of focused ultrasound on human-induced pluripotent stem cell (HiPSC) neurons.

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