Using Inducible Osteoblastic Lineage-Specific <em>Stat3</em> Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Yuanqi Liu; Siyuan Sun; Ziyi Jiang; Xinyi Gong; Yiling Yang; Yanfei Zhu; Hongyuan Xu; Anting Jin; Xiangru Huang; Xin Gao; Tingwei Lu; Jingyi Liu; Xinyu Wang; Qinggang Dai; Lingyong Jiang

doi:10.3791/65613

Utilisation de souris knock-out Stat3 inductibles spécifiques à la lignée ostéoblastique...

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JoVE Journal Medicine

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Utilisation de souris knock-out Stat3 inductibles spécifiques à la lignée ostéoblastique pour étudier le remodelage osseux alvéolaire pendant le mouvement des dents orthodontiques

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05:25 min

July 21, 2023

DOI: 10.3791/65613-v

Yuanqi Liu*^1,2,3,4,5, Siyuan Sun*^1,2,3,4,5, Ziyi Jiang*⁶, Xinyi Gong^1,2,3,4,5, Yiling Yang^1,2,3,4,5, Yanfei Zhu^1,2,3,4,5, Hongyuan Xu^1,2,3,4,5, Anting Jin^1,2,3,4,5, Xiangru Huang^1,2,3,4,5, Xin Gao^1,2,3,4,5, Tingwei Lu^1,2,3,4,5, Jingyi Liu^1,2,3,4,5, Xinyu Wang^1,2,3,4,5, Qinggang Dai^2,3,4,5,7, Lingyong Jiang^1,2,3,4,5

¹Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine, ²College of Stomatology,Shanghai Jiao Tong University, ³National Center for Stomatology, ⁴National Clinical Research Center for Oral Diseases, ⁵Shanghai Key Laboratory of Stomatology, ⁶Shanghai Starriver Bilingual School, ⁷The 2nd Dental Center, Ninth People’s Hospital, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine

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Overview

This study investigates the mechanism of bone remodeling under mechanical force, specifically during orthodontic tooth movement (OTM). Using inducible osteoblast lineage-specific Stat3 knockout mice, the research aims to elucidate the effects of mechanical stimuli on alveolar bone remodeling.

Key Study Components

Area of Science

Bone remodeling
Orthodontics
Mechanical biology

Background

Bone remodeling is influenced by mechanical forces.
Orthodontic tooth movement induces significant bone changes.
Previous studies focused on gene expression and cellular functions.
Tracing specific gene functions in vivo during OTM is challenging.

Purpose of Study

To explore the role of specific genes in bone remodeling during OTM.
To utilize a time-efficient model for studying mechanical effects on bone.
To enhance understanding of skeletal mechanical biology for clinical applications.

Methods Used

Inducible osteoblast lineage-specific Stat3 knockout mice.
Analysis of alveolar bone remodeling.
Assessment of mechanical force impacts on bone.
Comparative analysis with other experimental models.

Main Results

Identification of gene functions during OTM.
Insights into the mechanisms of bone response to mechanical stimuli.
Demonstration of the effectiveness of the knockout mouse model.
Contribution to the understanding of skeletal biology.

Conclusions

The study provides a novel approach to studying bone remodeling.
Findings may inform future clinical treatments in orthodontics.
Highlights the importance of specific genes in mechanical bone responses.

Frequently Asked Questions

What is the significance of using knockout mice in this study?

Knockout mice allow researchers to specifically investigate the role of certain genes in bone remodeling during orthodontic tooth movement.

How does orthodontic tooth movement affect bone remodeling?

OTM induces mechanical forces that lead to significant changes in alveolar bone structure and remodeling processes.

What methods were used to analyze bone remodeling?

The study utilized histological analysis and imaging techniques to assess changes in alveolar bone during OTM.

What are the potential clinical implications of this research?

Understanding the genetic mechanisms of bone remodeling could lead to improved orthodontic treatments and strategies for managing bone health.

What challenges exist in studying bone remodeling in vivo?

Tracing specific gene functions during bone remodeling in live subjects presents significant technical and methodological challenges.

Why is it important to study the effects of mechanical force on bone?

Studying mechanical forces on bone helps to elucidate the biological processes involved in bone health and disease, informing better treatment approaches.

Cette étude fournit un protocole pour l’utilisation de souris knock-out Stat3 inductibles spécifiques à la lignée ostéoblastique pour étudier le remodelage osseux sous force orthodontique et décrit des méthodes d’analyse du remodelage osseux alvéolaire pendant le mouvement orthodontique des dents, mettant ainsi en lumière la biologie mécanique squelettique.

Nos recherches portent sur le mécanisme de remodelage osseux sous l’effet d’une force mécanique, incluant l’os alvéolaire et les os longs. Nous essayons de répondre à la façon dont les os réagissent aux stimuli mécaniques afin d’apporter un nouvel éclairage sur la biologie mécanique du squelette pour un traitement clinique ultérieur. À l’heure actuelle, les études sur l’OTM se limitaient à l’analyse de l’expression génique in vivo ou à l’analyse de la fonction cellulaire in vitro.

À cette fin, un défi s’est présenté. Comment tracer et explorer la fonction de gènes spécifiques dans des lignées spécifiques au cours de l’OTM in vivo ? C’est pourquoi nous utilisons ici des souris knock-out conditionnelles inductibles.

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