Un système de perfusion normanthropique open-source conçu pour les chercheurs
1.9K vues
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Cité par 2
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11:23 min
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18 juillet 2025
DOI :10.3791/68722-v
18 juillet 2025
•1.9K vues
Lindsay R. Friedman1 , Hannah E. Stepp1 , Jacob Lambdin1 , Allen Luna1 , Alexander Rossi1 , Stephanie C. Lux1 , Michael M. Wach1 , Kenneth Luberice1 , Samantha M. Ruff1 , James McDonald1 , Reed I. Ayabe1 , Shahyan U. Rehman1 , Carrie Ryan1 , Stephanie Gregory1 , Alyssa V. Eade1 , Carolina Larrain1 , Jack H. Victory1 , Randy Pursley2 , Taylor Noel2 , Max Freidlin2 , Shreya Gupta1 , Tracey Pu1 , Ashley Rainey1 , Emily Verbus1 , Martha Teke1 , Areeba Saif1 , Tahsin M. Khan1 , Imani A. Alexander1 , Surajit Sinha1 , Sarfraz R. Akmal1 , Ritish R. Maram3 , Leila Sarvestani1 , Stephen M. Hewitt4 , James Doroshow5 , Kirsten Remmert1 , Emily Smith1 , Priyanka Desai1 , Ning Miao6 , Kevin Driscoll6 , Kamille West7 , Theo Heller8 , Elliot Levy3 , Jeremy Davis1 , Andrew Blakely1 , David E. Kleiner4 , John Kakareka2 , Thomas Pohida2 , Jonathan M. Hernandez1,9
1Surgical Oncology Program, National Cancer Institute, National Institutes of Health, 2Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 3Radiology and Imaging Sciences, Department of Interventional Radiology, National Institutes of Health, 4Histopathology and Autopsy Pathology, Laboratory of Pathology, National Institutes of Health, 5Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, 6Department of Perioperative Medicine, National Institutes of Health, 7Blood Services Section, Department of Transfusion Medicine, National Institutes of Health, 8Translational Hepatology Section, Liver Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9Center for Immuno-Oncology, National Cancer Institute, National Institutes of Health
Ici, nous présentons un protocole pour un système de perfusion ex vivo normothermique modifiable open-source, conçu pour surmonter le coût élevé et la flexibilité limitée des plateformes existantes, permettant une utilisation plus accessible, rentable et itérative dans la recherche biomédicale préclinique.
Chapters in this video
0:00
Introduction
0:50
Machine Set-Up Normothermic Ex Vivo Perfusion System
9:29
Results
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