Question 1

What are telomeres and why do they shorten during DNA replication?

Accepted Answer

Telomeres are repetitive DNA sequences at chromosome ends that protect genetic material from degradation. During DNA replication, the lagging strand synthesis cannot fully replicate the 5' end of linear chromosomes, causing telomeres to shorten with each cell division. This progressive shortening limits cellular replication capacity and triggers aging or senescence.

Question 2

How does telomerase solve the end-replication problem?

Accepted Answer

Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosome ends, compensating for losses during replication. It uses an internal RNA template to synthesize new telomeric DNA sequences, effectively extending chromosome length. This mechanism allows cells to maintain telomere length across multiple divisions and is particularly active in germ cells and cancer cells.

Question 3

What is the relationship between telomere length and cellular aging?

Accepted Answer

Telomere length serves as a molecular clock for cellular aging. As telomeres progressively shorten with each division, cells eventually reach a critical length threshold, triggering the Hayflick limit where cells stop dividing. This telomere-driven senescence is a key mechanism linking DNA replication dynamics to organismal aging and age-related disease.

Question 4

Why is telomerase activity elevated in cancer cells?

Accepted Answer

Cancer cells reactivate telomerase to bypass the Hayflick limit and achieve unlimited replication potential. Approximately 85-95% of cancers express telomerase, enabling indefinite cell division. This telomerase reactivation is a critical step in malignant transformation, allowing tumors to grow without the normal telomere-imposed replication constraint.

Question 5

How do telomeres protect chromosome stability?

Accepted Answer

Telomeres form specialized protein-DNA complexes that shield chromosome ends from recognition as double-strand breaks. This protective cap prevents inappropriate DNA repair mechanisms from fusing chromosome ends together. Loss of telomeric protection triggers genomic instability and can activate DNA damage responses that halt cell cycle progression.

Question 6

What structural proteins are associated with telomeres?

Accepted Answer

Telomeres are bound by shelterin complex proteins, including TRF1, TRF2, and POT1, which recognize and protect telomeric DNA sequences. These proteins prevent chromosome ends from being recognized as DNA breaks and regulate telomerase access. The shelterin complex is essential for maintaining telomere function and genomic stability throughout the cell cycle.

Question 7

How does telomere dysfunction contribute to disease?

Accepted Answer

Critically short telomeres or defective shelterin complexes trigger DNA damage responses and cell cycle arrest, leading to tissue dysfunction and premature aging syndromes. Telomere dysfunction is implicated in cardiovascular disease, pulmonary fibrosis, and bone marrow failure. Conversely, inappropriate telomerase reactivation drives cancer development and progression.

טלומרים וטלומראז

Browse More Videos