Question 1

What triggers GPCR desensitization in cells?

Accepted Answer

Prolonged exposure to high ligand concentrations triggers GPCR desensitization to prevent receptor overstimulation. When ligand-bound GPCRs continuously activate G proteins, the cell initiates feedback mechanisms to reduce signaling. This allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when ligands are no longer needed.

Question 2

How does beta-arrestin inactivate GPCRs?

Accepted Answer

Beta-arrestin binds to phosphorylated GPCRs and blocks additional G proteins from attaching to the receptor, directly inactivating it. Phosphorylation by GRK or PKA creates binding sites for beta-arrestin on the receptor's cytosolic domain. This competitive binding prevents downstream signaling activation, effectively switching off the receptor's response to ligands.

Question 3

What is the difference between homologous and heterologous desensitization?

Accepted Answer

Homologous desensitization occurs when specific GRKs phosphorylate and inactivate only a particular ligand-bound GPCR, such as beta-adrenergic receptor kinase targeting beta-adrenergic receptors. Heterologous desensitization happens when PKA phosphorylates multiple Gαs-coupled receptors, including those not directly stimulated by the original ligand, causing cross-regulation across different receptor types.

Question 4

How does receptor sequestration contribute to GPCR desensitization?

Accepted Answer

Beta-arrestin recruits clathrin and adaptor proteins to phosphorylated GPCRs, triggering clathrin-mediated endocytosis that packages receptors into vesicles. These vesicles transport receptors to endosomes, making them inaccessible to extracellular ligands. Eventually, dephosphorylated receptors recycle back to the plasma membrane for another round of signaling.

Question 5

What role does GRK play in GPCR phosphorylation?

Accepted Answer

G protein-coupled receptor kinases phosphorylate serine and threonine residues on the cytosolic domain of activated GPCRs. This phosphorylation promotes beta-arrestin binding, which blocks G protein activation and inactivation of proteins. GRKs are recruited to the plasma membrane by free beta-gamma subunits released during G protein activation and inactivation of G proteins.

Question 6

What happens to GPCRs during receptor downregulation?

Accepted Answer

During receptor downregulation, beta-arrestin-bound GPCRs are ubiquitinylated and transported to lysosomes instead of recycling to the plasma membrane. Lysosomal enzymes degrade the ubiquitinylated receptors, permanently reducing the number of available receptors on the cell surface. This process allows cells to adjust receptor density in response to prolonged ligand exposure.

Question 7

How does PKA mediate feedback regulation of GPCR signaling?

Accepted Answer

Protein kinase A is activated downstream of Gαs-coupled receptors like beta-adrenergic and glucagon receptors. PKA then phosphorylates serine and threonine residues on these same receptors, preventing them from binding and activating additional G proteins. This feedback mechanism allows end products of signaling pathways to inhibit upstream receptors and prevent excessive cellular responses.

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