Question 1

What is apoptosis and why is it important for maintaining tissue balance?

Accepted Answer

Apoptosis is regulated cell death that eliminates aged or injured cells while maintaining tissue balance. During apoptosis, cells shrink, chromatin condenses and fragments, and the cell breaks into membrane-bound apoptotic bodies. Phagocytes then engulf these bodies without triggering inflammation, making apoptosis a clean removal process essential for embryonic development, hormone withdrawal, immune regulation, and viral infection responses.

Question 2

How do caspases and BCL-2 family proteins regulate apoptosis?

Accepted Answer

BCL-2 family proteins, including BAX, regulate pro- and anti-apoptotic signaling by controlling mitochondrial membrane permeabilization. When triggered, BAX promotes the release of cytochrome c from mitochondria. Cytochrome c then activates caspases, which function as executioner enzymes that dismantle the cell through controlled degradation of cellular components.

Question 3

What is autophagy and how does it support cell survival?

Accepted Answer

Autophagy is an intracellular recycling process that maintains cellular quality at baseline and is upregulated during stress to promote survival. The process begins with phagophore formation, which develops into an autophagosome that fuses with a lysosome for digestion. Autophagy sustains metabolism during starvation, clears damaged organelles, and supports overall cellular quality control.

Question 4

What are the structural changes that occur during apoptosis?

Accepted Answer

During apoptosis, cells undergo characteristic structural changes including cell shrinkage, chromatin condensation and fragmentation, and membrane blebbing. The cell then breaks apart into membrane-bound apoptotic bodies containing intact organelles and fragmented DNA. These apoptotic bodies are rapidly recognized and engulfed by phagocytes, preventing leakage of cellular contents and inflammation.

Question 5

How does autophagy differ from apoptosis in terms of cellular outcome?

Accepted Answer

Apoptosis is a form of programmed cell death that eliminates cells entirely, while autophagy is a survival mechanism that recycles damaged components to maintain cellular function. Apoptosis removes aged or injured cells to maintain tissue balance, whereas autophagy breaks down and recycles cellular material to sustain metabolism and support overall cell quality control during stress.

Question 6

What triggers apoptosis in response to cellular damage and stress?

Accepted Answer

Apoptosis can be triggered by severe cellular injury, endoplasmic reticulum stress, or caspase activation through intrinsic and extrinsic pathways. When cells experience damage beyond repair capacity, pro-apoptotic signals activate BCL-2 family proteins and mitochondrial pathways. Inadequate regulation of apoptosis contributes to diseases such as cancer and autoimmune disorders.

Question 7

How do cells decide between apoptosis and autophagy when facing stress?

Accepted Answer

Cells respond to damage and stress through tightly regulated processes that promote survival or initiate self-destruction. Autophagy is typically activated first during mild stress to maintain cellular quality and sustain metabolism. If stress persists or damage is severe, apoptotic pathways are triggered through intrinsic and extrinsic mechanisms to eliminate the compromised cell and prevent inflammation.

פגיעה תאית V: אפופטוזיס ואוטופגיה

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