Automated Priming
Automated Priming - Related Videos
06:16
Verbal Priming
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human memory seems to work in two broad ways. Like modern computers, the human mind has explicit, or declarative, memory: ask a question, and a person gives the best answer they can. Input a query, and a computer program returns the contents of the relevant parts of its stored memory. Humans also have a second kind of memory system, one not really typical of computers, one that experimental psychologists call implicit. Implicit...
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
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Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
13:54
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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Cited by 2 •
2013
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
09:34
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
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Cited by 1 •
2016
This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.
04:00
A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis
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2025
This video demonstrates a method to generate a mouse model of primed mycobacterial uveitis (PMU) for studying post-infectious uveitis. Upon induction of PMU by intravitreal injection of heat-killed Mycobacterium tuberculosis in a mouse previously immunized with the same immunogen, the progression of chronic inflammation in the eye is analyzed using imaging and histopathological...
FAQs
Coordination allows the system to execute fluid delivery, valve switching, and monitoring according to a predefined sequence rather than relying on separate manual actions. Pumps move specified fluids, valves direct them through selected paths, and sensors help establish controlled timing or pressure conditions. This integration makes preparation more repeatable across runs and supports reliable operation of complex fluidic systems.
Air remaining in channels or tubing can prevent complete fluid delivery and interfere with stable flow. Automated priming addresses this risk by using programmed fluid movement and controlled operating conditions to displace air before the experiment or process begins. In microfluidic, analytical, and bioprocessing systems, this preparation helps produce more consistent operation and reduces incomplete delivery.
Predefined fluid volumes determine how much of the system is filled or conditioned, while timing coordinates the order and duration of each preparation step. Pressure conditions help establish stable movement through channels and tubing. Controlling these variables together reduces run-to-run differences and gives the system a more consistent starting state for downstream analysis, cell handling, or bioprocessing.
Automated priming standardizes preparation through programmed sequences, coordinated components, and predefined operating parameters. Manual preparation can depend more heavily on how an operator performs each step, which may increase variability between experiments. By reducing that dependence, automation supports reproducible fluid delivery and helps limit errors associated with incomplete preparation, inconsistent conditioning, or uncontrolled timing.
A typical cycle programs the pumps and valves to introduce predefined fluid volumes into selected channels or tubing, while sensors and control settings support the intended timing and pressure conditions. The sequence may fill flow paths, displace air, condition relevant surfaces, and establish stable flow. Completing these steps before operation creates a controlled starting condition for the system.
Bioengineering applications include microfluidic devices, bioreactors, cell-handling platforms, and automated assays. In each setting, preparation must be consistent before fluids, cells, or analytical processes are handled. Automation is particularly useful when systems contain multiple flow paths or must perform repeated operations, because standardized preparation can reduce operator variability and support more reliable experimental workflows.
Standardized priming protocols provide a repeatable preparation step that can be integrated into larger automated workflows. Consistent filling, air displacement, surface conditioning, and flow establishment help experiments begin from comparable system states. This consistency supports integration across components, repeated assay operation, and the transition toward scale-up or high-throughput processing while limiting variability caused by manual preparation.