Peptide Delivery
Peptide Delivery - Related Videos
10:30
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Cited by 10 •
2015
We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.
04:00
Immunoaffinity Based Extraction of Ubiquitinylated Peptides: A Technique to Selectively Extract Ubiquitin Tagged Peptides from Purified Peptide Fractions
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2023
This video describes a method to extract and purify ubiquitinylated peptides containing remnant di-glycine peptides from a complex peptide mixture. The presented method may help in identifying original ubiquitination sites in the protein.
14:28
Peptide-based Identification of Functional Motifs and their Binding Partners
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Cited by 2 •
2013
Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.
03:51
Treatment of Rat Coronal Slices with a Neurotoxic Peptide
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2025
This video demonstrates the procedure to prepare and treat rat brain hemislices with a neurotoxic peptide to mimic neurodegeneration. The protocol allows for analyzing neurodegenerative events by directly comparing control and treated slices in the same anatomical plane.
03:21
Peptide Purification: An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates
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2023
In this video, we demonstrate reversed-phase high-performance liquid chromatography to purify peptides from a digested protein lysate mixture. These purified peptides are then lyophilized and stored for downstream applications. Analysis of peptides can help understand the mechanics involved in cellular signaling and cancer.
FAQs
Encapsulation places the peptide within a protective delivery system, while chemical conjugation offers a chemical strategy for modifying delivery. Nanoparticle-based carriers provide a platform that can be designed around stability, cellular uptake, or release behavior. Comparing these options helps bioengineers match system design to the peptide’s biological requirements.
Peptide therapeutics may lose effectiveness through enzymatic breakdown, leave circulation quickly, or enter cells inefficiently. These are distinct barriers, so improving one does not necessarily resolve the others. A useful design therefore considers protection from degradation, persistence in circulation, and cellular uptake together. This bioengineering perspective connects carrier selection with the biological location where the peptide must act.
Controlled release regulates the timing of peptide availability rather than allowing the payload to become available immediately. When combined with systems designed for tissue or cellular targeting, it can help coordinate exposure with the intended site of action. This matters because delivery performance depends not only on reaching a biological destination, but also on preserving activity and controlling availability there.
Design begins by identifying the intended tissue or cell and the barriers that could limit performance, including degradation, rapid clearance, or inefficient membrane crossing. Researchers can then consider encapsulation, chemical conjugation, or nanoparticle carriers in relation to the desired stability, uptake, and release behavior. This sequence keeps the delivery system aligned with the therapeutic peptide’s biological function.
It is especially valuable when a peptide must reach a defined tissue or cell while retaining biological activity. In targeted therapy, the system addresses transport and treatment precision; in vaccine development and diagnostics, it supports delivery of peptide-based biological components; and in regenerative medicine, it helps manage peptide availability in approaches aimed at tissue repair.
Successful systems are judged by whether they improve stability, uptake, controlled release, and delivery precision without losing biological activity. These outcomes provide a practical framework for interpreting a design: protection addresses degradation, uptake addresses cellular entry, and release control addresses timing and location. Together, they help bioengineers assess whether a carrier meaningfully improves efficacy and safety.