Bioreactor Flow Circuit
Bioreactor Flow Circuit - Related Videos
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
0 Views •
Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
12:54
Operation of a Benchtop Bioreactor
0 Views •
Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
0 Views •
2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
0 Views •
Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
0 Views •
Cited by 8 •
2015
A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Here, the HDBR is successfully applied in a photobioreactor (PBR) configuration for the study of nitrogen metabolism by a mixed high density algal community.
FAQs
Pump output supplies the driving force for medium movement, while resistance within the circuit influences pressure and circulation behavior. Together, these factors determine how consistently fluid reaches different reactor regions and how cells or engineered tissues experience transport conditions. Adjusting their relationship can help limit poorly supplied areas and support more reproducible cultivation.
Pressure and flow sensors provide information about the operating state of the circuit rather than relying only on the pump setting. When connected to feedback controls, they can help maintain selected conditions as transport changes. This monitoring supports reproducibility, identifies deviations during cultivation, and helps keep fluid exposure within the intended operating range.
Circulation patterns determine whether nutrients and gases reach culture regions uniformly and whether some areas receive excessive or insufficient fluid movement. Design therefore requires attention to both distribution and shear, the physical force produced by moving fluid. Balancing these effects helps reduce stagnant regions while avoiding unwanted conditions for cells or engineered tissues.
Planning should account for the vessels, tubing, pump, sensors, and control points needed to move and monitor culture medium. The arrangement should support uniform exposure, practical sampling, and control of pressure and flow. Considering these elements together can reduce stagnant regions and make the cultivation process more consistent and easier to evaluate.
This approach is useful when researchers need controlled medium transport during cell culture, tissue engineering, or bioprocess development. It allows circulation, sampling, and operating conditions to be managed as part of the reactor system. The same design principles also support scale-up, where reproducible transport becomes important across larger or more complex cultivation setups.
A controlled circuit can help researchers assess whether culture regions receive consistent exposure to nutrients and gases, whether sampling is efficient, and whether operating conditions remain reproducible. These observations are relevant to cultivation performance and reactor development. In tissue engineering and bioprocess work, they also help connect fluid transport design with the behavior of the biological system.