Cardiomyocyte Contraction
Cardiomyocyte Contraction - Related Videos
05:06
Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
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Cited by 4 •
2020
We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
11:02
Isolation and Physiological Analysis of Mouse Cardiomyocytes
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Cited by 25 •
2014
Individual cardiomyocytes from wild type and mutant mice can be isolated from the heart in order to study their contractility and calcium transients. This allows characterization of the contribution of cellular dysfunction to heart dysfunction from any cause.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
13:42
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
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Cited by 3 •
2013
The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.
FAQs
Electrical stimulation opens calcium channels, which raises the concentration of Ca2+ in the cytosol. This increase links the electrical event to the contractile machinery by providing the signal required for calcium to bind troponin. The resulting activation allows the cell to convert an electrical input into force production and sarcomere shortening.
Calcium binding changes the regulatory state of troponin, which shifts tropomyosin away from actin’s myosin-binding sites. Myosin can then form cross-bridges with actin and generate force. This regulatory sequence is important because it controls when the contractile proteins engage, coordinating activation rather than allowing continuous interaction between actin and myosin.
Relaxation begins when calcium is removed from the cytosol. As calcium levels fall, troponin no longer maintains the activated regulatory state, and tropomyosin returns to a position that limits actin-myosin interaction. The contractile proteins therefore disengage, ending force generation and allowing the sarcomere to return from its shortened state.
Researchers can examine contraction in isolated cardiomyocytes, engineered heart tissues, and organ-on-chip models. These systems support assessment of cardiac development, disease-related dysfunction, and electrical or mechanical performance. Comparing contraction behavior across model types helps bioengineers study how cellular activity scales to organized cardiac tissue and evaluate responses to potential therapies.
Engineered heart tissues and organ-on-chip models are useful when researchers need to evaluate cardiomyocyte behavior in designed cardiac environments rather than only in isolated cells. Contraction measurements in these systems can reveal tissue-level electrical and mechanical performance, support studies of cardiac development and dysfunction, and provide a setting for examining potential therapeutic responses.
Contraction measurements provide functional information about how cardiomyocytes respond to electrical stimulation and how effectively they generate and release force. In bioengineering studies, these readouts can help identify disease-related dysfunction, characterize cardiac development, and assess the performance of engineered tissues or organ-on-chip systems. They can also support evaluation of potential therapies.