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Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
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Medicine
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JoVE Journal Medicine
Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

DOI:
10.3791/61104-v

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05:06 min

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May 05, 2020

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, , , , , ,
1Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University, 2Institute of Laboratory Animals, Graduate School of Medicine,Kyoto University

Chapters

  • 00:04Introduction
  • 01:05Human Induced Pluripotent Stem Cell (hiPSC) Cardiac Differentiation Induction
  • 02:30Induced Pluripotent Stem Cell-Cardiomyocyte (iPS-CM) Contractility Assessment
  • 03:15Ischemia Exposure
  • 03:42Results: Representative Cardiac Cell Differentiation
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

Tags

Keywords: IPS CellsCardiomyocytesIschemic Heart DiseaseVideo AnalysisCardiomyocyte ContractionHuman Heart PhysiologyPersonalized Drug ScreeningHiPSC-derived CardiomyocytesParticle Image VelocimetryPhase Contrast Microscopy

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