Microinjection Skills
Microinjection Skills - Related Videos
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
12:09
Generation of Stable Transgenic C. elegans Using Microinjection
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Cited by 93 •
2008
This video demonstrates the technique of microinjection into the gonad of C. elegans to create transgenic animals.
04:34
Scientific Measurement and Lab Skills - Concepts
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2020
Two-Dimensional Graphs Some chemistry experiments take the form of directly changing one property of the system that you are studying otherwise known as the independent variable, like temperature, and measuring the effects on another property, otherwise known as the dependent variable, like volume. Once the data have been collected, the interaction between the two parameters must be quantified — or converted into a form that can be evaluated — and compared to other relationships.
05:12
Using Virtual Reality to Transfer Motor Skill Knowledge from One Hand to Another
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Cited by 11 •
2017
We describe a novel virtual-reality based setup which exploits voluntary control of one hand to improve motor-skill performance in the other (non-trained) hand. This is achieved by providing real-time movement-based sensory feedback as if the non-trained hand is moving. This new approach may be used to enhance rehabilitation of patients with unilateral hemiparesis.
12:10
Microinjection of Xenopus Laevis Oocytes
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Cited by 14 •
2009
Here we demonstrate cytoplasmic microinjection of Xenopus laevis oocytes with a nuclear import substrate, as well as preparation of the injected oocytes for visualization by thin-sectioning electron microscopy.
FAQs
Successful microinjection depends on coordinating three elements: microscopic visualization, a fine glass needle, and controlled micromanipulator movement. Visualization identifies the target and guides needle entry, while the micromanipulator provides the positional precision needed for penetration. Controlled pressure then releases a minute volume, helping limit physical disruption and supporting more consistent manipulation across samples.
The balance between penetration and delivery is central to the technique. The needle must enter the target far enough to release material, but the manipulation should avoid excessive damage. Pressure and movement therefore function together rather than independently: movement positions the needle, and pressure controls release. This coordination is especially important when working at the single-cell level.
The material selected determines what biological question the experiment can address. DNA or RNA delivery can support transgenesis and gene-editing workflows, whereas proteins or cellular components enable other forms of direct cellular manipulation. Because the material enters a defined microscopic target, investigators can examine or modify biological activity with greater spatial and cellular control.
A basic workflow begins with microscopic guidance of the target, followed by positioning a fine glass needle with a micromanipulator. The needle penetrates the selected cell, embryo, or other microscopic target, and controlled pressure releases the intended material. Consistency depends on maintaining coordinated positioning and delivery while avoiding excessive damage during the manipulation.
In bioengineering, these skills are useful when an experiment requires precise manipulation of a cell or embryo rather than a general treatment of a population. Applications described for the technique include transgenesis, gene-editing workflows, embryo manipulation, and studies of cell function. The method consequently supports engineered cell models and research involving regenerative medicine and development.
Microinjection can provide a direct route for testing how introduced materials or cellular components affect a biological system. At the embryo level, it contributes to manipulation relevant to developmental studies; at the cell level, it supports investigation of cell function and engineered models. Its principal outcome is controlled, single-cell access, which can improve experimental consistency when performed reliably.