Human Ige Fc-receptors
Human Ige Fc-receptors - Related Videos
03:32
An In Vitro Artificial Activation Assay for Studying Fc Receptor Activation by Antibodies
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2025
This video demonstrates FcγRIIIa-driven events initiated by therapeutic antibodies in human natural killer cells. The artificial stimulation platform facilitates the exploration of downstream effector functions, encompassing cytoskeletal rearrangement, degranulation, chemokine/cytokine production, and signaling pathways mediated by the FcγRIIIa and Fc portions of antibodies involved in binding.
09:40
Detection of True IgE-expressing Mouse B Lineage Cells
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Cited by 3 •
2014
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.
10:22
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Cited by 14 •
2011
Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.
10:33
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Cited by 3 •
2014
Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
03:03
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies
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2025
This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
FAQs
The critical step is the cross-linking of IgE molecules already associated with FcεRI by an allergen. This receptor clustering initiates tyrosine-kinase activity and receptor phosphorylation, which transmit an intracellular activation signal. The resulting response includes release of inflammatory mediators, making cross-linking a central mechanism connecting allergen recognition with allergic inflammation.
FcεRI and FcεRII contribute to IgE biology through different functional patterns. FcεRI is associated mainly with mast-cell and basophil activation after allergen-driven cross-linking, whereas FcεRII, also called CD23, influences IgE transport, uptake, and immune regulation. Comparing these receptors helps distinguish immediate inflammatory signaling from broader control of IgE handling.
Tyrosine kinases and receptor phosphorylation provide the signaling events that follow receptor engagement. Their activity converts allergen-induced receptor cross-linking into downstream cellular activation, ultimately supporting inflammatory mediator release. Measuring or characterizing these events can therefore help researchers determine whether a stimulus affects receptor signaling itself rather than only IgE binding or receptor abundance.
Flow cytometry can characterize receptor expression on relevant cell populations, including the mast cells and basophils associated with FcεRI-mediated responses. By examining receptor-associated signals across cells, researchers can compare expression patterns and assess how experimental conditions relate to receptor presence. This makes the technique useful for linking cellular phenotype with IgE-dependent immune behavior.
Binding assays examine interactions between receptors and their ligands, helping characterize IgE or other relevant ligand interactions. Antibody-based methods can support detection or analysis of receptor expression and associated binding properties. Used alongside flow cytometry, these approaches provide complementary information about receptor abundance, ligand recognition, and the relationship between binding and signaling.
These receptors are examined when researchers need to investigate allergy, asthma, host defense, therapeutic antibodies, or strategies targeting IgE-dependent inflammation. The experimental focus may be receptor expression, signaling, or ligand interaction, depending on the question. Together, these measurements help connect cellular receptor behavior with broader immune outcomes and potential intervention strategies.