Bacterial Feeding

Bacterial feeding is the process by which bacteria obtain nutrients and energy needed for growth, reproduction, and cellular maintenance. Depending on the species and environment, bacteria secrete extracellular enzymes to break complex materials into soluble molecules, then absorb them through membrane transporters; others generate energy through photosynthesis or the oxidation of inorganic compounds. These strategies allow bacteria to occupy diverse habitats and drive decomposition, carbon and nutrient cycling, and symbiotic interactions. Understanding bacterial feeding supports research in ecology, microbiology, biotechnology, soil fertility, wastewater treatment, and the development of methods for manipulating microbial communities.

Bacterial Feeding - Related Videos

Research

JoVE EoE - Drosophila melanogaster (fruit fly)

Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster

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2023

This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

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2020

This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

Transfection of Wild-Type Amoeba via Electroporation with Bacterial Feeding Support

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2025

Source: Paschke, P., et al. Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria. J. Vis. Exp. (2019).This video demonstrates the procedure for introducing a plasmid carrying a target gene into amoeba cells via electroporation, followed by recovery in a bacterial supplement to enable efficient gene delivery in wild-type amoeba.

Research

JoVE Journal - Environment
Free Sample

Assaying Predatory Feeding Behaviors in Pristionchus and Other Nematodes

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Cited by 18 •

2016

Behavioral assays provide powerful tools for understanding neuronal function. Here we present several protocols for quantifying predatory feeding behavior found in the model nematode Pristionchus pacificus and its relatives. Additionally, we provide methods for analyzing predatory feeding adaptations including mouth structures and teeth.

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

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Cited by 19 •

2016

Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.

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