Cell Plate Formation

Cell plate formation is the process by which a dividing plant cell builds a new partition between daughter cells during cytokinesis, enabling tissue growth and organization. After chromosome separation, the phragmoplast guides Golgi-derived vesicles to the cell’s equatorial region, where the vesicles fuse to form a membranous cell plate that expands outward and connects with the parental cell wall. The developing plate matures into a new cell wall and separates the daughter-cell plasma membranes. Understanding this process is essential for studying plant development, cell wall assembly, and the mechanisms that maintain tissue structure.

Cell Plate Formation - Related Videos

Research

JoVE Journal - Biology

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

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Cited by 10 •

2013

Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.

Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

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Cited by 6 •

2017

The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

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Cited by 105 •

2010

We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

Preparation of a Microfluidic Plate with Bacterial Cells

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2025

Source: Sutlief, A. L., et al. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (2019).This video demonstrates the preparation of a microfluidic plate for shear stress studies in bacterial cultures. After priming the channels with media, bacterial cells are introduced into the experimental channel and incubated for attachment. Residual media and culture are removed to prepare the system for subsequent shear...

Research

JoVE Journal - Immunology and Infection
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Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

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Cited by 29 •

2011

Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.

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