Dna Viruses
Dna Viruses - Related Videos
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
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Cited by 129 •
2010
Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.
13:22
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Cited by 12 •
2014
We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.
21:55
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
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Cited by 35 •
2012
We demonstrate the basic technique to molecularly engineer and evolve synthetic Adeno-associated viral (AAV) gene therapy vectors via DNA family shuffling. Moreover, we provide general guidelines and representative examples for selection and analysis of individual chimeric capsids with enhanced properties on target cells in culture or in mice.
08:56
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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2018
Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
09:31
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Cited by 53 •
2016
We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using BEDTools.
FAQs
Replication can rely on host enzymes, viral DNA polymerases, or a combination of both. This division of labor affects how the viral genome is copied after entry and helps explain why DNA viruses are biologically diverse. Comparing enzyme dependence can therefore support genome analysis and improve understanding of interactions between viral replication systems and susceptible host cells.
Genome release makes the viral DNA available to the cellular environment, where replication and viral protein production can begin. The outcome depends on whether the cell provides suitable conditions and enzymes, whether the virus supplies additional replication machinery, and how effectively newly produced components are assembled. These factors shape the progression of infection.
Persistent or latent infections can continue to affect biological systems beyond the initial phase of viral production. Their study helps researchers examine long-term host–pathogen interactions rather than focusing only on immediate cell damage or virion release. This perspective is important for understanding disease processes and for developing approaches that address infections that do not simply end after one replication cycle.
Genome analysis provides a way to examine the organization and characteristics of viral DNA, supporting classification within a diverse group. Researchers can relate genome features to replication strategies, protein production, and interactions with host cells. These comparisons also contribute to studying viral evolution, because differences and similarities in genomes help clarify relationships among viruses.
Research on DNA viruses connects viral genome analysis and infection biology with practical disease-control goals. Understanding how genomes are copied, how proteins are produced, and how virions leave cells can identify features relevant to diagnosis and intervention. The same knowledge supports development of antiviral approaches and vaccines designed around important aspects of the infection process.
DNA viruses can serve as viral vectors for gene delivery, making them useful tools in biological research as well as subjects of disease investigation. Their study also provides insight into host–pathogen interactions, viral evolution, and the consequences of persistent infection. Thus, work on these viruses can inform both experimental gene-delivery strategies and broader studies of cellular biology.