Journal
/
Neuroscience
/
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

DOI:
10.3791/51091-v

•

13:22 min

•

January 23, 2014

•

, , ,
1Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire,CNRS UMR 5534, 2Université de Lyon 1, 3Laboratoire d’excellence,LabEX DEVweCAN, 4Institut de Virologie Moléculaire et Structurale,CNRS UPR 3296, 5Centre de Recherche en Cancérologie de Lyon, INSERM U1052,CNRS UMR 5286

Chapters

  • 00:05Title
  • 02:27Animal Infection
  • 03:20Trigeminal Ganglia Harvesting and Sectioning
  • 05:02DNA-FISH
  • 10:08Protocol Development and Applications
  • 12:32Conclusion

Summary

Automatic Translation

Automatic Translation

We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.

Tags

Keywords: DNA-FISHRNA-FISHImmunofluorescenceHSV-1LatencyGene Expression RegulationSingle Cell Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51091?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/51091?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/51091?article_type=v

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
check_url/51091?article_type=v

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
check_url/51091?article_type=v

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
check_url/51091?article_type=v

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
check_url/51091?article_type=v

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
check_url/51091?article_type=v

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
check_url/51091?article_type=v

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
check_url/51091?article_type=v

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Read Article