Glucan-mediated Adhesion
Glucan-mediated Adhesion - Related Videos
02:25
An Assay for Analyzing Test β-Glucan as an Immunotherapy Strategy against Brain Cancer Cells
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2025
This video demonstrates an in vitro assay utilizing β-glucans to activate microglial cells for the production of superoxide radicals, a reactive oxygen species. In brain cancer cells, the generated reactive oxidants actively suppress tumor cell proliferation and induce cell death.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
07:40
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Cited by 3 •
2016
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
09:11
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Cited by 4 •
2015
Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.
03:25
Cytoskeleton and Focal Adhesion Organization Assay: An Immunofluorescence-based Method to Study Cell Adhesion and Spreading on Substrates
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2023
This video describes the technique of studying the cytoskeleton and focal adhesions in primary human colon cancer cells to understand its variations depending on substrate rigidity. This culture of cancer cells on soft and hard substrates allows various downstream biophysical measurements for cancer prognosis.
FAQs
Glucans can function at different stages depending on where they are found. Cell-wall glucans contribute directly to cell-surface interactions, whereas released glucans become part of an extracellular matrix. That matrix can support both attachment and cell-cell cohesion, helping explain how microbial communities remain associated with a surface rather than dispersing.
The chemical properties of glucans influence how they interact with surfaces and with other matrix components. These interactions can promote initial binding, strengthen connections between neighboring cells, and retain additional material within the extracellular matrix. Consequently, glucan composition and organization can affect the stability and structure of a developing biofilm.
Adhesion and cohesion provide complementary functions in biofilm development. Glucans help cells remain attached to a surface while also linking cells to one another through the surrounding matrix. By retaining other matrix components, they can influence community structure and create a more persistent microbial arrangement.
Researchers examine which factors support cell attachment, whether glucans are displayed on the cell wall or released into the extracellular matrix, and how those locations relate to cohesion and material retention. These observations help connect glucan-associated interactions with microbial persistence, biofilm organization, and the ability to remain associated with a surface.
This research can identify glucan-associated factors that support attachment and persistence, creating targets for approaches intended to reduce surface binding or weaken community cohesion. Evaluating such strategies is relevant because limiting glucan-dependent interactions may alter biofilm formation, reduce retention of matrix components, and provide insight into how microbial colonization might be disrupted.
In biology, glucan-mediated interactions are especially relevant to fungal biofilms and host-associated infections. Studying them helps clarify how extracellular polymers support colonization and persistence in these settings. The same framework also connects cell-surface attachment with broader questions about microbial community structure and resistance to environmental stress.