Nanoflow Electrospray Ionization
Nanoflow Electrospray Ionization - Related Videos
08:43
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
0 Views •
Cited by 39 •
2009
We describe a new quantitative lipidomics method for identifying numerous lipid species in yeast using survey-scan electrospray ionization mass spectrometry (ESI/MS). This method exceeds currently available methods for lipid identification and quantification in the ability to resolve various molecular forms of lipids, sensitivity, and speed.
12:59
A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
0 Views •
2016
The characterization of complexes formed in different relative ratios of mercury(II) to dicysteinyl tetrapeptides by electrospray ionization orbitrap mass spectrometry is presented.
01:12
Electrospray Ionization (ESI) Mass Spectrometry
0 Views •
2024
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule. ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
0 Views •
Cited by 21 •
2013
Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue sections.
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
0 Views •
Cited by 19 •
2016
A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.
FAQs
Applying high voltage to a narrow emitter creates a Taylor cone, which produces a fine spray of charged droplets. Solvent evaporation progressively concentrates the material until analyte ions are released into the gas phase and transferred to the mass spectrometer. This sequence connects emitter behavior and desolvation directly to the availability of biological molecules for mass analysis.
The low flow rate can improve ionization efficiency and detection sensitivity, allowing researchers to examine biological samples when only limited material is available. This feature is especially valuable in proteomics, metabolomics, and lipidomics, where the amount of peptide, protein, metabolite, or lipid mixture may constrain how much sample can be introduced for analysis.
The narrow emitter concentrates the liquid flow at the point where the applied voltage acts, supporting formation of the Taylor cone and a fine charged spray. That spray provides the starting material for solvent evaporation and ion release. Consequently, emitter geometry is closely tied to how the liquid becomes compatible with mass spectrometric measurement.
In biological analysis, nano-ESI commonly operates with liquid chromatography-mass spectrometry. Liquid chromatography supports analysis of complex molecular mixtures, while the electrospray stage converts separated liquid-phase components into ions for mass spectrometric detection. This combined workflow enables sensitive examination of biological constituents rather than treating the sample as a single undifferentiated mixture.
Nano-ESI supports proteomics, metabolomics, and lipidomics. In these areas, researchers analyze peptides and proteins, metabolites, or lipids within biological samples and complex mixtures. Its low-flow operation is particularly useful when sample amounts are limited, while the resulting ionization and detection support molecular characterization across these distinct areas of biology.
The technique provides gas-phase ions that a mass spectrometer can detect, supporting characterization of peptides, proteins, metabolites, and other complex molecular mixtures. In biological research, these measurements help investigators examine the molecular composition of limited samples and apply the resulting data to proteomic, metabolomic, or lipidomic investigations.