Vascular Ligation
Vascular Ligation - Related Videos
07:34
DNA Ligation Reactions
0 Views •
2023
In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular cloning to join DNA fragments of inserts with vectors – carrier DNA molecules that will replicate target fragments in host organisms. This video provides an...
11:53
Cecal Ligation Puncture Procedure
0 Views •
Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
0 Views •
Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
04:37
Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development
0 Views •
Cited by 3 •
2023
Here, we present a detailed visual protocol for executing the left atrial ligation (LAL) model in the avian embryo. The LAL model alters the intracardiac flow, which changes wall shear stress loading, mimicking hypoplastic left heart syndrome. An approach to overcome the challenges of this difficult microsurgery model is presented.
08:18
Engineered Vascularized Muscle Flap
0 Views •
Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
FAQs
The constricting material compresses the vessel wall and narrows or closes the lumen, reducing the passage of blood through that segment. This interruption limits leakage from the vessel and can establish hemostasis, meaning control of bleeding. The same physical change can also redirect circulation toward other available vascular pathways.
Tissues beyond the closed vessel may receive less blood because perfusion through the treated pathway has been reduced. This altered supply can affect local tissue responses and organ function. Over time, collateral vessels may compensate for the interruption, making ligation useful for examining how tissues respond to reduced or redirected circulation.
Collateral vessels provide alternative routes for blood flow after the primary pathway has been closed. Their ability to compensate helps determine whether downstream tissues continue to experience reduced perfusion or regain support from other vessels. Consequently, vascular ligation can reveal both the effects of interrupted flow and the capacity of circulation to adapt.
A vessel may be secured with a suture, clip, or another constricting material. Each option works by maintaining compression against the vessel wall so that the lumen remains occluded. In biological or biomedical procedures, the selected material supports the intended goal, such as controlling bleeding, preventing leakage, or changing circulation for an experimental comparison.
The procedure centers on placing a constricting material around the selected blood vessel and securing it tightly enough to compress the vessel wall and occlude its lumen. This establishes control over flow through that segment. The resulting change can then be used to manage bleeding or create a defined reduction or redirection of blood supply in a study.
Researchers use the technique in experimental models to examine vascular function, tissue perfusion, wound healing, and organ responses to altered blood supply. By closing a chosen vascular route, they can study how reduced or redirected circulation influences tissues and organs. The approach also provides a way to investigate responses associated with changes in blood flow.
During surgery, ligation helps control bleeding or prevent blood from escaping through a vessel. Securing the vessel with a suture, clip, or similar material closes its lumen and limits flow through the treated segment. This local control supports hemostasis and can also redirect circulation when the procedure requires blood flow to follow alternative pathways.