Air Cell Relocation
Air Cell Relocation - Related Videos
09:04
Fabrication of VB2/Air Cells for Electrochemical Testing
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Cited by 5 •
2013
A protocol is presented to study multi-electron metal/air battery systems by using previous technology developed for the zinc/air cell. Electrochemical testing is then performed on fabricated batteries to evaluate performance.
02:35
The Water Maze with Platform Relocation: A Method to Assess Working Memory
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2023
In this video, we describe the Morris water maze test with platform relocation, a navigational task used to study working memory in rodents.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 135 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
03:17
Viral Growth in Human Nasal Epithelial Cells Cultured at an Air-Liquid Interface
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2026
Source: Otter, C. J., et al. Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions. J. Vis. Exp. (2023)This video demonstrates the growth of coronavirus in human nasal epithelial cells cultured at an air-liquid interface, mimicking infection and viral replication in the human nasal cavity.
01:27
Air-entraining Agents
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2024
Air-entraining agents improve the durability and workability of concrete in climates with frequent freezing and thawing. These agents prevent cracks by introducing small air bubbles into the mix, creating spaces accommodating water expansion when temperatures drop. The air-entraining agents lower the surface tension of water, forming stable, small air bubbles. This method is more effective than having accidental large voids, as the intentional, smaller, and evenly distributed air voids improve...
FAQs
The technique requires shifting or creating the internal air space beneath a selected shell region without disrupting the conditions needed for embryo survival and CAM development. This balance is central to its success: researchers gain access for observation while preserving the living avian embryo environment that supports a functional, vascularized membrane for subsequent cancer studies.
Selecting the shell region determines where the observation window will provide practical access to the developing CAM. Positioning the air space beneath an appropriate area helps expose the membrane while maintaining the surrounding conditions required for continued development. This makes the preparation suitable for direct observation and later monitoring of implanted tumor material.
The CAM provides a vascularized, living setting in which researchers can examine cancer-related processes rather than relying only on isolated samples. Within this preparation, tumor cells or tissue grafts can be monitored for growth, angiogenesis, and invasion. Its accessibility also supports direct imaging and sampling as disease-related changes develop.
Preparation centers on identifying a suitable shell region, creating or shifting the egg’s internal air space beneath that area, and opening an observation window that exposes the CAM. Throughout the preparation, conditions must remain compatible with embryo survival and membrane development. The resulting setup allows tumor material and subsequent changes to be examined in place.
The preparation supports observation of tumor growth, formation of blood vessels associated with angiogenesis, tissue invasion, and responses to experimental treatments. Direct imaging can follow these changes during disease progression, while sampling provides material for further evaluation. Together, these outcomes connect visible changes in the living model with cancer-related biological behavior.
It is useful when a study requires a vascularized living model that remains accessible for repeated observation or sampling. After tumor cells or tissue grafts are introduced, researchers can follow progression and examine treatment responses within the developing embryo environment. This makes the approach relevant to studies of tumor biology, angiogenesis, invasion, and candidate therapies.