Ht29 Tumorspheres
Ht29 Tumorspheres - Related Videos
06:52
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
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Cited by 1 •
2020
Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.
09:21
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
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Cited by 2 •
2019
This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
02:44
In Vitro Gut Model to Assess Bacterial Adhesion and Internalization
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018).This video demonstrates an in vitro gut host–microbe model to study bacterial viability. A co-culture of Caco-2 and HT29-MTX cells is incubated with bacteria pre-treated under simulated digestive conditions. Epithelial barrier integrity is assessed using transepithelial electrical resistance, and fractions from the medium, solubilized...
09:44
Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior
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Cited by 6 •
2016
This manuscript describes an ex vivo model system comprised of organ-conditioned media derived from the lymph node, bone, lung, and brain of mice. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
FAQs
The culture conditions create a selection environment in which attachment-independent survival becomes important. Without serum and adhesion, only a subset of HT29 cells survives and proliferates, while EGF and bFGF support sphere growth. This enrichment helps investigators examine heterogeneity and stem-like behavior rather than treating the original cell population as functionally uniform.
After initial spheres form, researchers can dissociate them and place the resulting cells into fresh sphere-forming conditions. Repeated sphere generation provides a way to examine self-renewal, meaning the capacity to produce new sphere-forming populations over successive rounds. This approach helps distinguish transient survival in culture from a more persistent stem-like phenotype.
Following sphere formation, cells can be evaluated in differentiation studies to ask whether the enriched population retains the ability to produce different cellular states. Comparing sphere-derived cells with the starting HT29 population can add context to measurements of heterogeneity and stem-like behavior in colorectal cancer. The results complement observations based only on sphere growth.
Generation requires HT29 human colorectal cancer cells in non-adherent, serum-free culture conditions, with growth-factor supplementation such as EGF and bFGF. The non-adherent setting makes attachment-independent survival central to the experiment, while the supplemented medium supports proliferation of cells able to organize into spheres. These elements establish the environment for downstream analysis.
Researchers can compare sphere-forming behavior with characteristics of the starting population to investigate whether stem-like cells become enriched under the culture conditions. Sphere formation, serial sphere production, and differentiation studies provide complementary evidence. Together, these readouts support analysis of self-renewal and heterogeneity, while the model can also be used to examine tumorigenic potential.
The model supports investigation of colorectal cancer progression and therapeutic resistance. Because the system enriches a sphere-forming subset and permits analysis of responses to anticancer treatments, investigators can examine how stem-like tumor cell behavior relates to treatment resistance. Findings can be interpreted alongside measures of self-renewal, differentiation, and tumorigenic potential.