Flow Cell Testing
Flow Cell Testing - Related Videos
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
10:19
Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
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Cited by 9 •
2016
Design and fabrication of a three-dimensionally (3-D) printed microfluidic cross-flow filtration system is demonstrated. The system is used to test performance and observe fouling of ultrafiltration and nanofiltration (thin film composite) membranes.
11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Cited by 42 •
2016
Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.
09:49
Kupffer Cell Isolation for Nanoparticle Toxicity Testing
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Cited by 28 •
2015
Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.
FAQs
Residence time determines how long reactants or electrolytes remain in the reaction chamber before leaving it. Changing this duration can alter the extent of reaction, measured conversion, selectivity, and the opportunity for mass transfer to occur. Comparing results at controlled residence times helps researchers distinguish kinetic limitations from transport effects and identify operating conditions that improve performance.
Flow rate controls how quickly material passes through the defined reaction chamber and therefore influences residence time and transport behavior. If the rate changes, measured conversion, selectivity, and reaction performance may change even when concentration and temperature remain constant. Systematic flow-rate tests reveal whether performance is sensitive to throughput and help guide reactor or cell optimization.
Concentration, temperature, pressure, flow rate, and residence time are central variables because each can affect reaction behavior and movement through the chamber. Holding these conditions at controlled values makes comparisons reproducible, while varying them individually or in combination shows how the system responds. This approach supports identification of conditions that improve efficiency, stability, or desired product formation.
Measurements of conversion, selectivity, and mass transfer provide complementary views of system behavior. A change in flow conditions may alter how efficiently reactants or electrolytes move through the chamber, while temperature or concentration changes may affect the reaction itself. Examining these outcomes under controlled conditions helps researchers determine whether limited performance arises primarily from transport, reaction behavior, or their interaction.
A typical test establishes the reaction chamber and sets the selected flow rate, concentrations, temperature, pressure, and residence time. The system is then operated under controlled conditions while researchers measure reaction performance, mass transfer, conversion, selectivity, and stability. Repeating measurements across defined operating conditions creates comparable data for evaluating how the system responds.
The approach supports several chemistry applications, including flow reactors, electrochemical cells, fuel cells, and redox-flow batteries. In these systems, testing reveals how transport and reaction behavior respond to operating conditions. The resulting comparisons can inform reactor design, energy-efficiency improvements, and assessment of whether performance remains stable during extended operation.
Extended operation can show whether a chemical or electrochemical system maintains its reaction performance and stability under controlled flow conditions. Alongside conversion, selectivity, and mass-transfer measurements, these tests provide evidence about behavior over time rather than at a single operating point. Such data help evaluate designs, support scale-up decisions, and identify conditions associated with reliable performance.