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JoVE Journal
Immunology and Infection

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Kupffer Cell Isolation for Nanoparticle Toxicity Testing
 

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Article DOI: 10.3791/52989 09:49 min
August 18th, 2015

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Summary August 18th, 2015

Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.

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