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Biology

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Flow Cytometry Purification of Mouse Meiotic Cells
 

Flow Cytometry Purification of Mouse Meiotic Cells

Article DOI: 10.3791/2602
April 15th, 2011

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Summary April 15th, 2011

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

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