Pet-raft
Pet-raft - Related Videos
07:26
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
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Cited by 47 •
2012
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
10:54
Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization
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2015
An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
0 Views •
Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
10:35
Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia
0 Views •
Cited by 4 •
2015
The method presented here uses simultaneous positron emission tomography and magnetic resonance imaging. In the cerebral hypoxia-ischemia model, dynamic changes in diffusion and glucose metabolism occur during and after injury. The evolving and irreproducible damage in this model necessitates simultaneous acquisition if meaningful multi-modal imaging data are to be acquired.
FAQs
The photocatalyst absorbs light and transfers energy or an electron to create the radicals needed for polymer growth. Because this initiation is light-mediated, the chemical system can connect radical production to illumination rather than relying solely on uncontrolled radical formation. This photochemical control helps support more deliberate adjustment of polymer molecular architecture under relatively mild conditions.
The chain-transfer agent regulates growing polymer chains through reversible addition and fragmentation. These repeated exchanges limit uncontrolled growth, allowing chemists to guide molecular weight, composition, and functionality more carefully than an unregulated process would permit. Its role is therefore central to translating radical chemistry into polymers with planned architectures rather than broadly distributed, poorly controlled structures.
Light activates the photocatalyst, while the chain-transfer agent manages the resulting radical activity. These functions address different parts of the process: photochemistry supplies the initiating event, and reversible addition and fragmentation moderates chain growth. Together, they provide a mechanism for linking external light input with precision polymer design and for limiting the effects of uncontrolled radical propagation.
The method supports adjustment of polymer molecular weight, composition, and functionality. These variables determine how a polymer can be designed for a particular material purpose, including the construction of advanced coatings, biomaterials, nanostructures, and responsive systems. The key chemical advantage is not simply producing polymer, but obtaining molecular architectures whose properties can be deliberately varied.
A high-level workflow combines a polymer-forming chemical system with a photocatalyst and a chain-transfer agent, followed by light exposure. Illumination activates the photocatalyst, and the transfer agent regulates radical-driven growth. Chemists then use the controlled process to target a desired molecular weight, composition, functionality, or architecture under the relatively mild conditions associated with the method.
Researchers may choose PET-RAFT when they need greater control over polymer architecture while using a light-mediated process. Its ability to tune molecular weight, composition, and functionality makes it relevant to materials development rather than simple polymer formation alone. The approach is especially suited to projects involving coatings, biomaterials, nanostructures, or polymers that respond to external conditions.
PET-RAFT applies photochemical energy or electron transfer to the problem of precision polymer design. The photocatalyst responds to light, and reversible chain regulation converts that stimulus into controlled molecular growth. This connection allows chemists to develop polymer systems for coatings, biomaterials, nanostructures, and responsive materials, demonstrating how fundamental photochemistry can guide functional material architecture.