Weight Fraction Analysis
Weight Fraction Analysis - Related Videos
07:57
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
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Cited by 17 •
2009
The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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Cited by 217 •
2014
Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.
08:22
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
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Cited by 13 •
2015
Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.
17:06
Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
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Cited by 17 •
2012
We describe a novel approach for simultaneous analysis of brain function and structure using magnetic resonance imaging (MRI). We assess brain structure with high-resolution diffusion-weighted imaging and white-matter fiber tractography. Unlike standard structural MRI, these techniques allow us to directly relate anatomical connectivity to functional properties of brain networks.
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
FAQs
The total mass must represent the same mixture as the measured component mass. Using an incomplete sample, an inconsistent portion, or a total that excludes relevant material changes the calculated ratio and can misrepresent composition. This consistency is especially important when analyzing solutions, alloys, powders, or any mixture whose components are measured separately.
The decimal form expresses the component-to-mixture mass ratio directly, while the percentage form presents the same result on a 100-part basis. Converting between them makes composition easier to interpret or compare, but it does not change the measured composition. The preferred format depends on whether calculations, reporting, or mixture preparation is the primary goal.
Complete separation ensures that the measured component mass does not omit material or include an unseparated portion of another component. Drying helps remove material that could add mass without representing the component being quantified. Together, these steps improve the connection between the measured mass and the composition value calculated from it.
Balance precision affects both the component mass and the total sample mass used in the calculation. Small measurement differences can therefore influence the reported ratio, particularly when the relevant masses are close to the balance’s measurement limits. Careful weighing supports more reliable composition values for laboratory analysis and for calculations based on those values.
A typical workflow begins by measuring the sample and identifying the component of interest. The component is then separated as completely as possible, dried when needed, and weighed accurately. Finally, its mass is divided by the total sample mass, with the result reported as a decimal or converted to a percentage for interpretation.
The method is useful whenever composition needs to be expressed on a mass basis across different material types. In solutions, alloys, and powders, it provides a consistent way to describe how much of a selected component contributes to the mixture’s total mass. That information supports mixture preparation, quality control, and comparison of experimental samples.
A measured mass fraction connects the mass of a component with the total mass of the mixture. That relationship can help determine how much component is present in a selected sample amount, providing composition information for stoichiometric calculations. Reliable use depends on accurate masses, complete separation, appropriate drying, and a consistent total-mass basis.
They should first review whether the component was completely separated, whether drying was sufficient, and whether the component and total masses describe the same sample. Balance precision also deserves attention because measurement limitations can affect the ratio. Checking these factors helps distinguish a genuine composition result from an issue in sample handling or measurement.