Secretion Mobilization

Secretion mobilization is the clinical process of loosening and moving retained airway secretions so patients can clear mucus more effectively and maintain adequate ventilation. It uses approaches such as hydration, humidification, directed coughing, postural drainage, chest percussion, and oscillatory devices to reduce secretion viscosity, promote airflow behind mucus, and shift it toward larger airways. Clinicians select techniques according to the patient’s respiratory status, secretion characteristics, mobility, and ability to cough. Effective mobilization can improve airway clearance, reduce breathing effort, support gas exchange, and help prevent complications associated with secretion retention in acute and chronic respiratory conditions.

Secretion Mobilization - Related Videos

Education

JoVE Science Education - Chemistry

Electrophoretic Mobility Shift Assay (EMSA)

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2023

The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...

Mobile Marketing

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2024

As consumers increasingly rely on smartphones and other mobile devices for browsing, communication, and shopping, mobile marketing has become essential in today's digital world. Brands must adjust their strategies to engage users where they spend most of their time, which is on mobile screens. Understanding mobile user behavior is crucial for successful mobile marketing. Mobile users typically interact with their devices briefly and frequently throughout the day. They prefer content that is...

Research

JoVE Journal - Biology

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

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Cited by 11 •

2017

This work describes a fluorescence microscopy-based method for the study of platelet adhesion, spreading, and secretion under flow. This versatile platform enables the investigation of platelet function for mechanistic research on thrombosis and hemostasis.

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Cited by 2 •

2022

Ion mobility spectrometry (IMS) is an interesting complement to mass spectrometry for the characterization of biomolecules, notably because it is sensitive to isomerism. This protocol describes a tandem IMS (IMS/IMS) experiment, which allows the isolation of a molecule and the generation of the mobility profiles of its fragments.

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

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Cited by 7 •

2019

This protocol describes how to perform rapid low-cost luciferase assays at medium-throughput using an insulin-linked Gaussia luciferase as a proxy for insulin secretion from beta cells. The assay can be performed with most luminescence plate readers and multichannel pipettes.

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