3d Projection View
3d Projection View - Related Videos
11:34
High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
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Cited by 9 •
2013
This video describes the fundamentals of digital fringe projection techniques, which provide dense 3D measurements of dynamically changing surfaces. It also demonstrates the design and operation of a high-speed binary defocusing system based on these techniques.
12:45
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
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Cited by 10 •
2022
Tunneling nanotubes (TNTs) are primarily open-ended F-actin membrane nanotubes that connect neighboring cells, facilitating intercellular communication. The notable characteristic that distinguishes TNTs from other cell protrusions is the hovering nature of the nanotubes between cells. Here, we characterize TNTs by constructing a 3D volume view of confocal z-stack images.
02:18
Fischer Projections
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2023
Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines. While...
16:44
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
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Cited by 15 •
2009
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
02:06
Newman Projections
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2023
Different notations are used to represent the three-dimensional structure of molecules on two-dimensional surfaces. One of the most commonly used representations is the dash-wedge formula. The dashed wedges, solid wedges, and the plane lines indicate the groups situated behind the plane, coming out of the plane, and in the plane, respectively. The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as conformers.
FAQs
Depth-dependent projection combines signals from different positions within an optical stack rather than requiring each section to be viewed separately. This arrangement allows features distributed through the specimen to appear together in one image while retaining their relationships within the volume. For developmental biology, that integrated view can make cell distributions and anatomical organization easier to interpret.
The projected image can preserve meaningful information about the specimen’s three-dimensional organization, including the relative arrangement of features across depth. Although the result is displayed on a two-dimensional surface, signals from sequential optical sections contribute to the view. This helps reveal how cells, tissues, or developing structures relate spatially within the original volume.
Viewing individual optical sections separates the specimen into depth-specific images, whereas a 3D Projection View brings information from the image stack into one composite view. The two approaches therefore support different interpretations: separate sections show the contents of particular levels, while the projection helps researchers recognize broader spatial relationships and anatomical patterns across the volume.
The workflow begins with a volumetric image stack made from sequential optical sections. Signals from those sections are then combined through a projection that accounts for their position through depth, producing a single two-dimensional representation. Researchers can inspect this image to interpret the specimen’s organization, compare developmental patterns, and communicate structures that would be harder to convey through separate sections alone.
It is useful when researchers need to examine embryos, tissues, or forming organs as spatially organized volumes. A projected view can bring cell distributions and changing anatomical patterns into a common image, supporting interpretation across developmental contexts. This makes the approach relevant when the relationships among structures are as important as the appearance of any individual optical section.
These views support image interpretation, comparative analysis, and communication of developmental processes. Researchers can use them to clarify spatial relationships within a specimen, compare anatomical patterns across samples or developmental situations, and present complex volumetric observations in a more accessible form. Their value lies in connecting image-stack information with questions about organization and change during development.