Adult Stem Cells
Adult Stem Cells - Related Videos
01:33
Adult Stem Cells
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2019
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
09:24
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
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Cited by 9 •
2016
Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.
13:21
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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Cited by 113 •
2010
This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.
09:44
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
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Cited by 7 •
2011
Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.
FAQs
Signals from the local stem cell niche help determine whether adult stem cells continue self-renewal or produce specialized progeny. This microenvironment therefore connects tissue conditions with cellular behavior. In developmental biology, examining niche signals helps explain how mature tissues maintain appropriate renewal while adapting their output to changing physiological needs or injury.
Asymmetric division produces two different outcomes from one cell division: one daughter retains stem cell properties, while the other begins differentiation. This arrangement supports continued replacement of specialized cells without exhausting the stem cell population. It provides a mechanism for coordinating long-term tissue maintenance with the generation of mature cell types.
The balance depends on coordinating repeated self-renewing divisions with the production of differentiated progeny. Too little renewal could impair tissue maintenance, whereas insufficient differentiation could limit the supply of specialized cells. Studying this balance gives developmental biologists a framework for understanding how tissues sustain normal function and respond to repair demands.
Comparing hematopoietic, intestinal, and neural stem cells allows researchers to examine how stem cell behavior supports renewal in distinct mature tissues. These systems provide developmental biology contexts for studying tissue-specific maintenance, maturation, and repair. Their differences can also help clarify whether disrupted regulation reflects a broader stem cell principle or a tissue-associated process.
Adult stem cell research supports disease modeling by providing systems in which disrupted regulation can be examined in relation to tissue behavior. The same field contributes to tissue engineering by informing how cell renewal and specialization might be coordinated. These applications connect developmental mechanisms with efforts to understand or construct functional tissue responses.
Adult stem cells are relevant because regulation of renewal, differentiation, and repair can change during aging or become disrupted in cancer. Studying these processes helps connect developmental biology with disease mechanisms. The resulting knowledge also supports regenerative medicine, where understanding tissue maintenance and injury responses is important for developing repair-oriented strategies.