Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Hassan Azari; Maryam Rahman; Sharareh Sharififar; Brent A. Reynolds

doi:10.3791/2393

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

JoVE Journal
Neuroscience
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Full Text

28,381 Views

13:21 min

November 20, 2010

DOI: 10.3791/2393-v

Hassan Azari^1,2, Maryam Rahman², Sharareh Sharififar², Brent A. Reynolds²

¹ Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , ²Department of Neurosurgery,University of Florida

This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

Overall goal of the following experiments is to isolate and expand adult mouse neuro stem cells using the neuro sphere assay. This is achieved by first obtaining adult mouse brains, followed by dissection of the brains under the microscope, removal of the septum and isolation of periventricular tissue. Then the tissue is minced and tryps inized to achieve single cell suspension.

The cells are then plated in neuro stem cell media for culture. Hi, my name is Hassan Ali and I'm working with Dr.Brent Reynolds at the University of Florida McKnight Brain Institute. Today I'm gonna demonstrate how to isolate and expand neural stem cells from the adult mouse brain.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos