Carcinogenesis Research
Carcinogenesis Research - Related Videos
06:53
Cell Population Analyses During Skin Carcinogenesis
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Cited by 6 •
2013
Carcinogenesis is a process involving interactions between cancer cells and the cancer microenvironment. To dissect the molecular events, one needs to isolate different cell populations at different stages during cancer development. Using a mouse model for basal cell carcinoma, we describe a protocol for cell analyses during carcinogenesis.
08:38
Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia
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Cited by 13 •
2013
We describe the manufacture of compressed hormone pellets, as well as subcutaneous surgical implantation into mice. This strategy can be combined with the growth of cell and tissue xenografts under the renal capsule of athymic nude mice to evaluate hormonal carcinogenesis and regulation of benign prostate growth.
03:02
Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer
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2023
This video describes the procedure to induce two-stage skin carcinogenesis to study tumor initiation by topical application of chemicals. In the first step, a mutagen, DMBA, is applied onto the skin of a mouse model which initiates tumor formation by causing mutations in the stem cells, followed by the application of a growth stimulator, TPA, which accelerates skin papilloma formation.
09:42
Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
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Cited by 19 •
2014
Small animal imaging techniques allow serial diagnostic examinations and therapeutic interventions in vivo. Recently, the scope of applications has significantly widened and currently includes assessment of colonic tumor development, wound healing and monitoring of inflammation. This protocol illustrates these diverse potential applications of murine endoscopy.
04:12
Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis
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2023
In this video, we demonstrate the implantation of human-derived prostate cancer tissue grafts into the renal capsule of an immunocompromised recipient mouse. This technique can be used to assess tumor progression and develop novel therapeutic strategies.
FAQs
Researchers examine genetic and epigenetic changes as complementary routes to altered cell behavior. These changes can disrupt cell-cycle regulation, DNA repair, differentiation, and communication with surrounding tissues. Studying their combined effects helps identify how normal cellular programs become deregulated and clarifies which alterations may contribute to cancer initiation or progression.
Developmental biology offers a comparison between tumor formation and embryonic growth or tissue patterning. Both contexts involve regulated signaling, changing cell states, and decisions about cell fate. By examining where these processes diverge, researchers can investigate how altered developmental programs, including abnormal stem-cell behavior, contribute to the emergence and organization of tumors.
The cellular microenvironment matters because cells do not regulate growth and identity in isolation. Carcinogenesis research examines how communication with surrounding tissues changes when cancer develops and how that altered context may influence cell fate, growth, and tumor progression. This perspective broadens analysis beyond changes within individual cells to include tissue-level controls.
These processes represent distinct safeguards that help maintain appropriate cell behavior. Research considers how disruption of cell-cycle regulation can affect proliferation, how altered differentiation can change cellular identity, and how impaired DNA repair can permit additional abnormalities to accumulate. Examining them together helps explain why multiple disrupted programs may shape cancer development rather than a single defect acting alone.
A comparative approach examines signaling, stem-cell behavior, cell fate, and communication with surrounding tissues in both tumors and developing tissues. Researchers use these parallels to identify developmental processes that may become misregulated during carcinogenesis. The comparison can reveal how tissue-patterning principles are altered and help connect cellular changes with the broader organization of a tumor.
By clarifying cancer-driving mechanisms and the ways tumors arise from disrupted developmental programs, this research can identify biologically meaningful features of disease. Those findings support the development of diagnostic strategies and targeted therapies. Developmental biology adds context by linking candidate disease mechanisms to signaling, stem-cell behavior, differentiation, and the surrounding cellular environment.