Ethyl Acetate Extraction

Ethyl acetate extraction is a liquid-liquid separation technique that uses ethyl acetate to isolate organic compounds from aqueous or environmental samples. The method relies on differences in solubility and polarity: when ethyl acetate contacts a water-based sample, target compounds partition between the immiscible organic and aqueous phases, which can then be separated, often with a separatory funnel. In environmental analysis, this process prepares water, soil, or other samples for chemical measurement by concentrating organic constituents and reducing matrix interferences. The extracted phase can subsequently undergo evaporation, purification, or instrumental analysis to assess pollutants and support environmental monitoring.

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Research

JoVE Journal - Biology

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

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Cited by 11 •

2011

A method for the determination of acetate kinase activity is described. This assay utilizes a direct reaction for determining enzyme activity and kinetics of acetate kinase in the acetate-forming direction with different phosphoryl acceptors. Furthermore, this method can be utilized for assaying other acetyl phosphate or acetyl-CoA utilizing enzymes.

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants

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Cited by 6 •

2017

A protocol for the synthesis of high purity nonsymmetric dialkylphosphinic acid extractants is presented, taking (2,3-dimethylbutyl)(2,4,4'-trimethylpentyl)phosphinic acid as an example.

Facile Preparation of (2Z,4E)-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate

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Cited by 1 •

2017

The ruthenium-catalyzed olefination of electron-deficient alkenes with allyl acetate is described here. By using aminocarbonyl as a directing group, this external oxidant-free protocol has high efficiency and good stereo- and regioselectivity, opening a novel synthetic route to (Z,E)-butadiene skeletons.

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

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Cited by 40 •

2012

We describe a method protocol for the GC-based analysis of the aldonitrile acetate derivatives of glucosamine and muramic acid extracted from soil. For elucidation of the chemical mechanism, we also present a strategy to confirm the structure of the derivative and the ion fragments formed upon electron ionization.

Temperature-programmed Deoxygenation of Acetic Acid on Molybdenum Carbide Catalysts

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Cited by 1 •

2017

Presented here is a protocol for the operation of a micro-scale temperature-programmed reactor for evaluating the catalytic performance of molybdenum carbide during acetic acid deoxygenation.

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