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Biology

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Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
 

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

Article doi: 10.3791/3474
December 19th, 2011

Summary December 19th, 2011

A method for the determination of acetate kinase activity is described. This assay utilizes a direct reaction for determining enzyme activity and kinetics of acetate kinase in the acetate-forming direction with different phosphoryl acceptors. Furthermore, this method can be utilized for assaying other acetyl phosphate or acetyl-CoA utilizing enzymes.

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