Embryo Electroporation
Embryo Electroporation - Related Videos
06:57
In ovo Electroporation of Chicken Embryos
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2023
Electroporation is a technique used in biomedical research that allows for the manipulation of gene expression via the delivery of foreign genetic material into cells. More specifically, in ovo electroporation is performed on early developing chicks (Gallus gallus domesticus) contained within their eggshells. In this procedure, DNA or knockdown constructs are first injected into a target tissue. However, the genetic material is unable to penetrate the plasma membrane to carry out its function...
06:10
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
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Cited by 2 •
2012
A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.
13:06
In Ovo Electroporations of HH Stage 10 Chicken Embryos
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Cited by 19 •
2007
Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.
09:38
Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
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Cited by 17 •
2016
We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.
19:04
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
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Cited by 13 •
2007
In-utero in-vivo injection and electroporation of the embryonic rat neocortex provides a powerful tool for the manipulation of individual progenitors ...
FAQs
Brief electrical pulses temporarily alter embryonic cell membranes by creating pores. Because DNA, RNA, and genome-editing reagents are charged, these openings allow them to cross into embryonic cells during the treatment window. The membrane disruption is transient, so the method depends on delivering the molecular cargo while pores are available and then allowing treated embryos to continue developing.
Timing is important because treatment occurs in early embryos, before development has progressed extensively. Genetic changes introduced at this stage can be carried into the embryo’s subsequent development, making it possible to examine how altered gene activity affects developmental outcomes. This early intervention also supports the generation of organisms or research models carrying introduced or edited genetic material.
DNA, RNA, and genome-editing reagents serve different experimental purposes within the same delivery framework. DNA can support transgenesis, while RNA or genome-editing reagents can support studies that modify gene activity or genetic information. Selecting among them therefore depends on whether the study aims to introduce genetic material, alter gene activity, or investigate the consequences of a targeted genetic change.
An Embryo Electroporation workflow begins with early embryos and the selected nucleic acid or genome-editing cargo. The material is placed in contact with the embryos, which then receive brief electrical pulses to promote cellular entry. After treatment, embryos are allowed to develop, and researchers can assess whether introduced or edited genetic material produces a resulting genetic or developmental outcome.
Researchers choose Embryo Electroporation when they need to manipulate genes at an early developmental stage in a model organism. It is useful for transgenesis, CRISPR-based genome editing, and functional studies of developmental genes. These applications let investigators compare development after a specific genetic change, helping connect gene function with observed phenotypes rather than examining gene activity in isolation.
The principal research outcome is a genetically altered embryo that can be followed as development proceeds. Analysis can focus on whether the embryo carries introduced material or an edit and on the phenotype associated with that change. In genetics, this provides a direct experimental route for testing developmental-gene function and can accelerate creation of research models.