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JoVE Journal
Developmental Biology

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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
 

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Article doi: 10.3791/54313
October 16th, 2016

Summary October 16th, 2016

We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.

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