Antigen Microarray
Antigen Microarray - Related Videos
05:04
Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen
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2025
In this video, we demonstrate the process for detecting antibody isotypes in multiple human serum samples using a microarray slide printed with antigenic subtypes of influenza virus strains.
10:16
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
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Cited by 9 •
2016
We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
03:31
A Technique to Detect Cyanobacterial Strains Using an Antibody Microarray Chip
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2025
This video demonstrates a method to detect the Cyanobacterial strains in an environmental sample using an antibody microarray chip. Samples carrying various bacteria interact with printed antibodies, and immunostaining with fluorophore-labeled anti-cyanobacterial-strain antibodies reveals the presence of specific cyanobacterial strains through fluorescence in distinct spots.
08:52
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
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Cited by 20 •
2019
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.
FAQs
Signal location and intensity connect an antibody response to a particular immobilized antigen. After the biological sample contacts the ordered surface, antibodies bind complementary targets, and labeled secondary antibodies generate spot-specific signals. Reading the pattern across many spots therefore reveals which antigens are recognized and supports comparison of response profiles rather than relying on a single antibody measurement.
Antigen choice determines what an array can reveal. Including multiple pathogen-associated or other antigens allows researchers to examine recognition across targets in the same sample, while the resulting pattern can expose antibody cross-reactivity. This makes the array useful for distinguishing a focused response from broader binding, although interpretation depends on the antigen panel selected.
Three components have complementary roles: immobilized antigens provide the binding targets, the serum or other biological sample supplies antibodies for testing, and labeled secondary antibodies make bound antibodies detectable. Keeping these functions conceptually separate helps explain why the measured signal reflects antigen recognition plus the detection step, rather than antibody presence alone.
A typical workflow begins by presenting a selected set of antigens in an ordered pattern on a solid surface. Serum or another biological sample is then brought into contact with the array so antibodies can bind complementary targets. Labeled secondary antibodies produce spot-specific signals, which are analyzed as a pattern across the array.
Profiles from samples associated with infection, vaccination, or pathogen exposure can be compared across the same antigen set. Differences in spot-specific binding patterns help characterize how immune responses vary between these contexts. The approach is especially useful when the research question concerns breadth of recognition, shared reactivity, or evidence of exposure rather than one isolated antibody-antigen interaction.
The resulting binding profiles can help identify candidate diagnostic markers, guide antigen selection for vaccines, and prioritize antigens for further investigation. In infection research, they also support serological studies and exposure assessment. These uses depend on interpreting the complete pattern of signals, because a single reactive spot does not describe the full antibody response or its cross-reactivity.