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JoVE Journal
Immunology and Infection

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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
 

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Article DOI: 10.3791/54543
September 15th, 2016

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Summary September 15th, 2016

We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.

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