Immature Dcs
Immature Dcs - Related Videos
03:47
Generation of Immature, Mature, and Tolerogenic Dendritic Cells from Monocytes
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2025
This video demonstrates the generation of immature, mature, and tolerogenic dendritic cells, or DCs, from human monocytes. The incubation of monocytes with growth factors and cytokines leads to the production of immature DCs. In response to immune regulatory molecules, immature DCs differentiate into tolerogenic and mature DCs.
06:09
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
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Cited by 37 •
2016
Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.
06:00
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice
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Cited by 17 •
2017
The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.
03:44
siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells
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2025
This video demonstrates a small interfering RNA (siRNA)-based technique to interfere with autophagic flux. A siRNA targeting FIP200 mRNA is introduced into monocyte-derived immature dendritic cells (iDCs) using electroporation, causing the knockdown of FIP200 expression and leading to the inhibition of nuclear lamin disruption via autophagy. Upon adding herpes simplex virus (HSV), the nuclear egress of viral progeny is inhibited due to the absence of lamin disruption via autophagic flux.
07:16
Minimum Volume Vitrification of Immature Feline Oocytes
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Cited by 10 •
2020
This manuscript describes a protocol for the minimum volume vitrification of immature cat oocytes with laboratory-made media on commercial supports. It covers every step from oocyte isolation from ex vivo gonads to vitrification and warming.
FAQs
They use several complementary uptake routes rather than relying on a single mechanism. Phagocytosis enables capture of larger particulate material, macropinocytosis continuously samples surrounding fluid, and receptor-mediated endocytosis internalizes material recognized through cell-surface receptors. This combination allows immature DCs to survey tissues broadly while acquiring both pathogen-derived and soluble antigens for later processing.
Pathogen-associated signals and damage-associated signals act as triggers for maturation. After recognition, dendritic cells process captured antigen more extensively, increase surface presentation, and migrate toward draining lymph nodes. These coordinated changes shift their role from tissue surveillance and antigen acquisition toward communication with naïve T cells and initiation of adaptive immune responses.
The relatively low surface expression of MHC class II and costimulatory molecules distinguishes the surveillance state from the T-cell-activating state. Immature cells are specialized for acquiring antigen, whereas maturation increases the molecular capacity needed for effective presentation and T-cell activation. This change is important when interpreting dendritic-cell behavior in tolerance, infection, and vaccine studies.
Antigen capture supplies material for processing, but it does not by itself describe the complete activation context. Recognition of pathogen-associated or damage-associated signals provides the indication that maturation should occur, leading to enhanced presentation and migration. Consequently, dendritic cells can connect what they encounter in tissues with the subsequent adaptive response in lymph nodes.
Their strong capacity to capture pathogens and soluble antigens makes immature DCs relevant to vaccine strategies focused on antigen delivery and presentation. Vaccine research can therefore consider both how antigen is acquired in tissues and how activating signals promote maturation, migration, and later stimulation of naïve T cells. These linked stages help explain how vaccine inputs may shape adaptive immunity.
During infection, their tissue-sampling activity supports early detection of pathogen-associated material before an adaptive response develops. Once relevant signals are recognized, maturation and migration to draining lymph nodes connect local antigen encounter with naïve T-cell activation. This places immature DCs at an important transition between frontline pathogen surveillance and the generation of a targeted immune response.
They provide a framework for examining how antigen capture and signal recognition influence whether immune responses are initiated or regulated. Their participation in pathogen surveillance, maturation, lymph-node migration, and T-cell activation links local tissue events to systemic immune outcomes. Accordingly, studies can use this cellular progression to investigate inappropriate immune activation as well as tolerance-related processes.