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JoVE Journal
Immunology and Infection

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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
 

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Article DOI: 10.3791/54128 06:09 min June 22nd, 2016
June 22nd, 2016

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Summary

Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.

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Generation Immature Dendritic Cells Mature Dendritic Cells Tolerogenic Dendritic Cells Metabolic Phenotypes Protocol Monocytes Vaccine Development Immunology Antigen Presentation In Vitro Monocyte Enrichment Peripheral Blood Mononucleal Cell Live Cells Cytometer CD-4 Micro Beads Staining Buffer Centrifuge Cell Pellet Medium Column Magnetic Field Separator
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