Monocyte Transfection
Monocyte Transfection - Related Videos
09:13
Isolation, Transfection, and Culture of Primary Human Monocytes
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Cited by 10 •
2019
Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
08:04
Neuronal Transfection Methods
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2023
Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them...
05:19
Visualization of Caspase Activation in Human Monocyte-Derived Macrophages
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2025
This video demonstrates the activation of caspase proteins in human macrophages using bimolecular fluorescence complementation reporter proteins. The pro-domains of caspase-1 are fused with non-fluorescent fragments of Venus proteins, which are recruited to inflammasome complexes. The proximity of inflammasomes induces the refolding and fluorescence of Venus fragments, confirming caspase activation in the macrophages.
FAQs
Carriers and electrical methods provide different routes for nucleic-acid entry, but monocytes may respond differently to each because they are sensitive to cellular stress. The selected approach must balance delivery efficiency with preservation of viability and immune function. This balance determines whether altered gene expression or protein production can be interpreted as a transfection effect rather than damage-related cellular dysfunction.
Monocytes can readily activate inflammatory pathways during manipulation, and that response may change cytokine production or other immune behaviors independently of the introduced nucleic acid. Monitoring inflammatory activation therefore helps distinguish intended molecular effects from procedure-associated effects. This distinction is especially important when studying cytokine regulation, host-pathogen interactions, or antimicrobial responses, where inflammation is itself a central experimental readout.
Exposure conditions are critical because excessive cellular stress can reduce viability or alter the functions being measured. Researchers therefore adjust the compatibility of the carrier or electrical method and control how cells encounter the transfection treatment. The goal is not simply to obtain nucleic-acid delivery, but to maintain monocytes capable of differentiating and responding in a biologically meaningful way.
Different nucleic-acid strategies support different experimental questions. Gene knockdown can reduce expression of a selected target, whereas reporter expression can provide a visible or measurable indication of regulatory activity. Comparing these outcomes with monocyte behavior helps connect a molecular pathway to cytokine regulation, antigen presentation, or responses to infection without treating all transfection results as equivalent.
A study typically begins by choosing the nucleic-acid objective, such as knockdown or reporter expression, followed by selection of a compatible carrier or electrical method. Exposure conditions are then controlled to preserve viability and immune-cell function. Researchers assess the resulting expression or protein production alongside cellular behavior, allowing delivery performance and biological consequences to be evaluated together.
Transfected monocytes are useful when researchers need to test how a defined molecular change affects host defense. The approach can support investigations of host-pathogen interactions, antiviral or antimicrobial responses, cytokine regulation, and antigen presentation. Because monocytes can differentiate into macrophages or dendritic cells, results may also help connect an introduced genetic change with later immune-cell behavior or function.