Mouse Holder Preparation
Mouse Holder Preparation - Related Videos
10:01
En Face Preparation of Mouse Blood Vessels
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Cited by 20 •
2017
A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.
18:02
Preparation of Dissociated Mouse Cortical Neuron Cultures
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Cited by 146 •
2007
This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.
14:06
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
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Cited by 26 •
2012
Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.
04:32
Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
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2023
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
08:47
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Cited by 52 •
2010
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
FAQs
Its main contribution is standardization. Preparing the device and positioning each mouse in a consistent orientation limits differences in body movement and access to the intended site. This reduces procedural variation, helping researchers apply interventions or collect samples more consistently during comparable procedures across experimental groups.
Alignment places the intended site in an accessible, consistent position while maintaining appropriate orientation. This matters because inoculation, blood collection, and tissue sampling depend on reaching the planned site reliably. Consistent access supports comparable dosing or sampling, making differences in measured immune responses or infection-related outcomes easier to assess across groups.
It establishes a repeatable relationship between the mouse, the restraint device, and the intended procedural site. With body movement limited and alignment maintained, the operator can perform the planned intervention or collect the planned sample under comparable conditions. This consistency supports more precise dosing or sampling and strengthens comparisons of experimental measurements.
Preparation starts by readying the restraint device, then positioning the mouse so its body is appropriately aligned. The holder should limit movement while preserving access to the intended procedural site. Maintaining the same preparation approach across animals helps standardize inoculation, collection, or sampling procedures and reduces differences caused by handling.
The essential setup consists of a restraint device prepared for use and a mouse positioned within it. Preparation should establish appropriate alignment and access to the intended site while limiting body movement. Keeping this arrangement consistent across animals supports comparable inoculation, blood collection, tissue sampling, or other interventions in immunology and infection studies.
A prepared holder is useful when an experiment requires stable positioning for inoculation, blood collection, tissue sampling, or another intervention. Standardized handling can improve reproducibility and support more consistent assessment of immune responses and infection-related outcomes. It is particularly relevant when researchers compare dosing, samples, or measurements across experimental groups.