Skin Inflammation Assay
Skin Inflammation Assay - Related Videos
02:49
A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation
0 Views •
2025
This video demonstrates an in vivo technique to assess a test antigen by inducing skin inflammation in a murine model. The test antigen—a hapten in an oil/acetone solution—is topically applied to the skin of the mouse ear, and inflammation of the skin is assessed after an adequate period.
03:46
Malassezia-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model
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2025
This video demonstrates Malassezia-induced skin infection in mice. Malassezia oil suspension is applied over the wounded ear, triggering inflammation and swelling of the ear tissue. Later, the ears are dissected, homogenized, and spread on an agar plate for growth. The number of colonies indicates the fungal burden resulting from skin inflammation.
07:50
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
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Cited by 39 •
2012
Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.
01:38
Inflammation
0 Views •
2019
Overview In response to tissue injury and infection, mast cells initiate inflammation. Mast cells release chemicals that increase the permeability of adjacent blood capillaries and attract additional immune cells to the wound or site of infection. Neutrophils are phagocytic leukocytes that exit the bloodstream and engulf invading microbes. Blood clotting platelets seal the wound and fibers create a scaffold for wound healing. Macrophages engulf aging neutrophils to end the acute inflammatory...
07:10
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
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Cited by 25 •
2013
We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.
FAQs
The initiating stimulus helps determine which inflammatory features become most informative. An irritant may emphasize tissue damage or barrier disruption, whereas a pathogen-associated stimulus or inflammatory mediator can highlight cytokine release, immune-cell activation, or changes in gene expression. Matching the stimulus to the research question allows investigators to distinguish general inflammation from responses relevant to infection or inflammatory skin disease.
Skin cells, tissue samples, and reconstructed skin models provide different experimental contexts for observing immune activity and tissue injury. A cellular system can support focused measurements of inflammatory signaling, while a tissue or reconstructed model can additionally reveal barrier disruption and broader tissue responses. Selecting the model therefore determines which aspects of inflammation the assay can represent and quantify.
Several complementary readouts can characterize the response, including cytokine release, immune-cell activation, barrier disruption, and altered gene expression. Cytokine measurements indicate inflammatory signaling, immune-cell activation reflects changes in cellular behavior, and barrier or gene-expression changes provide additional evidence of tissue effects. Using more than one readout can connect molecular inflammation with functional skin damage.
A basic workflow begins by selecting an appropriate skin cell, tissue, or reconstructed skin model and exposing it to an irritant, pathogen-associated stimulus, or inflammatory mediator. Researchers then quantify selected outcomes, such as cytokine release, immune-cell activation, barrier disruption, or gene-expression changes. The workflow should align the model, stimulus, and measurements with the biological question under investigation.
In infection-focused studies, the approach can reveal how skin responds to pathogen-associated stimulation and how inflammatory activity relates to tissue effects. Measurements of cytokines, immune-cell activation, barrier disruption, and gene expression provide different views of the host response. This information can clarify mechanisms connecting immune activity, tissue injury, and the skin’s interaction with infectious challenges.
Researchers can apply the method to examine anti-inflammatory drugs, antimicrobial treatments, and skin-protective formulations in models showing inflammatory or infection-related responses. Comparing measured cytokine release, immune-cell activation, barrier disruption, or gene-expression changes can indicate whether a treatment alters immune activity or limits tissue damage. Such results support investigation of therapeutic responses and disease mechanisms.