Hypoxia Model
Hypoxia Model - Related Videos
09:54
A Model to Simulate Clinically Relevant Hypoxia in Humans
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Cited by 1 •
2016
Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO2).
12:03
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
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Cited by 6 •
2016
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
10:41
A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids
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2026
Self-assembling human cardiac organoids (hCOs) are an emerging system for modeling human cardiac development and disease. Here, a methodology for generating and injuring hCOs with hypoxia-reoxygenation and details of expected histological and functional outcomes after injury are provided.
07:48
Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
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Cited by 16 •
2015
With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.
06:01
A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia
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Cited by 8 •
2015
Thromboembolic stroke models are vital tools for optimizing the recanalization therapy. Here we report a murine thrombotic stroke model based on transient cerebral hypoxic-ischemic (tHI) insult, which triggers thrombosis and infarction, and responds favorably to tissue plasminogen activator (tPA)-mediated fibrinolysis in a therapeutic window similar to those in stroke patients.
FAQs
Reduced oxygen availability limits oxygen-dependent prolyl hydroxylation, a modification that normally contributes to hypoxia-inducible factor degradation. When this process is restricted, hypoxia-inducible factors become stabilized and can drive downstream cellular responses. This mechanism allows investigators to connect an imposed oxygen condition with changes in gene expression, metabolism, angiogenesis, inflammation, and cell survival.
A controlled low-oxygen chamber changes the oxygen environment directly, whereas a chemical mimetic reproduces hypoxia-related signaling without relying on the same environmental manipulation. Both approaches can stabilize hypoxia-inducible factors by limiting oxygen-dependent prolyl hydroxylation and subsequent protein degradation. The choice therefore affects how researchers create the experimental condition while targeting a related cellular response.
Useful response categories include altered gene expression, metabolic changes, angiogenesis, inflammation, and cell survival. Examining several categories helps researchers determine whether oxygen deprivation mainly changes cellular regulation, energy use, vascular growth, inflammatory behavior, viability, or a combination of these outcomes. These measurements also provide mechanistic context for disease-related studies and therapeutic investigations.
Researchers need to specify the experimental system and the way reduced oxygen will be produced. The system may consist of cells, tissues, or organisms, while the oxygen condition can be created with a controlled low-oxygen chamber or a chemical mimetic. Aligning these choices with the response being studied improves interpretation of gene, metabolic, inflammatory, survival, or angiogenic outcomes.
These models let investigators examine how oxygen deprivation affects cells, tissues, or organisms in disease contexts involving inadequate oxygen availability. In ischemic injury and cardiovascular disease research, measured changes can include gene expression, metabolism, inflammation, angiogenesis, and cell survival. Such findings help characterize oxygen-related pathology and evaluate strategies intended to improve oxygen delivery or modify hypoxia-driven responses.
Cancer and respiratory disorders are among the medical settings in which altered oxygen availability and hypoxia-driven responses are relevant. Experimental systems allow researchers to study associated changes in metabolism, gene expression, angiogenesis, inflammation, and survival within a controlled context. The same approach can also support evaluation of therapies designed to modify these responses or improve oxygen delivery.