Metabolic Activity
Metabolic Activity - Related Videos
01:27
Isothermal Microcalorimetry to Monitor Metabolic Activity in Bacteria
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2025
Take serial dilutions of a bacterial culture and add aliquots into calorimetric vials containing growth medium.Load the sealed vials into the multi-well plate of an isothermal microcalorimeter to measure metabolic heat production.The device is held at a constant temperature to ensure the detected heat originates solely from bacterial metabolism.As bacteria metabolize nutrients and proliferate, they release heat that sensors detect in real time.In parallel, incubate identical vials in an...
03:10
An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity
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2025
Source:Uju L. Madu1, Nozethu Mjokane1, Maphori Maliehe1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.The video demonstrates a technique for evaluating the influence of photodynamic treatment (PDT) with a photosensitizer on the metabolic activity of murine macrophages. The cells undergo treatment with the photosensitizer followed by exposure to UV light, initiating PDT. This process triggers the photosensitizer to generate reactive oxygen species...
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
Breakdown pathways process carbohydrates, fats, and proteins in ways that support energy production, whereas assembly pathways use available resources to form cellular building blocks. These directions are functionally linked: the products and energy generated by one set of reactions can support the other. Examining this balance helps researchers interpret how cells maintain function or show altered physiology.
Enzymes regulate the reactions that make up metabolic pathways, helping control how nutrients are processed and how cellular molecules are assembled. ATP acts as an energy carrier, capturing usable energy and distributing it where cellular work requires it. Together, enzymatic regulation and ATP handling determine how effectively cells convert chemical resources into functional outputs.
Carbohydrates, fats, and proteins enter different aspects of cellular processing, so the nutrient being examined can influence which reactions and outcomes are most relevant. Considering the nutrient source helps distinguish energy-related activity from the production of cellular building blocks. This distinction is important when studying how cells respond to altered physiology or disease-associated changes.
Measurements can provide information about whether tissue is functioning as expected or showing altered physiology. They may also help characterize disease processes by detecting changes in the chemical activity that supports cellular work. Because metabolic measurements reflect tissue behavior rather than only structural appearance, they can contribute to assessment, research, and monitoring across medical contexts.
In these disorders, metabolic assessments help investigate changes in the reactions that sustain cells and in the energy-related functions associated with mitochondria. The measurements can support evaluation of altered physiology and disease processes, providing a way to connect cellular chemistry with medical investigation. They are therefore relevant to both mechanistic research and clinical assessment.
Cancer biology can use differences in metabolic activity to investigate altered cellular physiology, while imaging applications use metabolic measurements to assess tissue-related activity. These assessments may also help monitor responses to treatment. In personalized therapy, metabolic information can contribute to tailoring evaluation or management to the biological characteristics and treatment behavior of an individual’s disease.